ArticleFrontiers in medicine2019
Selection of a Real-Time PCR Housekeeping Gene Panel in Human Endothelial Colony Forming Cells for Cellular Senescence Studies.
Article in Frontiers in medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Un-biased housekeeping gene panel selection for high-validity gene expression analysis.Scientific reports · 2022Pooled it
- Single-cell RNA sequencing reveals the dysfunctional characteristics of PBMCs in patients with type 2 diabetes mellitus.Frontiers in immunology · 2024Trial
- Identification of a novel real-time PCR reference gene panel for EA.hy926 endothelial cells in different growth States and with DHA treatment.Scientific reports · 2026Article
- Comparative analysis of BMP signaling in human ECFCs reveals vascular bed-dependent ALK2 expression.Vascular biology (Bristol, England) · 2026Article
- Methods to Study CCT Expression and Impact on Cytoskeleton.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A Comprehensive Multiomics Signature of Doxorubicin-Induced Cellular Senescence in the Postmenopausal Human Ovary.Aging cell · 2025Article
- Identifying optimal reference genes for real-time quantitative polymerase chain reaction in human myocardial tissues.Cardiovascular research · 2024Article
- Dyrk1a is required for craniofacial development in Xenopus laevis.Developmental biology · 2024Article
- How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology.Frontiers in bioengineering and biotechnology · 2024Article
- Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages.BMC genomics · 2022Article
- Expansion of a novel population of NK cells with low ribosome expression in juvenile dermatomyositis.Frontiers in immunology · 2022Article
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- Environmental Exposures and Lung Aging: Molecular Mechanisms and Implications for Improving Respiratory Health.Current environmental health reports · 2021Review
- Rational, Unbiased Selection of Reference Genes for Pluripotent Stem Cell-Derived Cardiomyocytes.Tissue engineering. Part C, Methods · 2021Article
- Alterations of housekeeping proteins in human aged and diseased hearts.Pflugers Archiv : European journal of physiology · 2021Article
- Identification of the best housekeeping gene for RT-qPCR analysis of human pancreatic organoids.PloS one · 2021Article
- ERgene: Python library for screening endogenous reference genes.Scientific reports · 2020Article
- Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.BMC genetics · 2020Article
- Effect of tobacco smoking on the epigenetic age of human respiratory organs.Clinical epigenetics · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endothelial Colony Forming Cells (ECFCs) represent a subset of endothelial progenitors with well-documented vasoreparative capacity. However, cellular senescence, which occurs due to aging, diabetes, smoking, or tissue inflammation, renders these cells dysfunctional. Therefore, there is growing interest in studying expression of senescence markers in ECFCs. RT-qPCR is the most commonly used technique to quantify gene expression and the proper choice of reference genes used for data normalization is critical for accurate quantification. It has been reported that the expression of commonly used housekeeping genes is often unstable in senescence. To identify the most suitable reference genes for ECFC senescence studies, we analyzed a microarray dataset, which compared the gene expression between proliferating and senescent ECFCs. In addition to replicative senescence, the data included X-ray-induced and Etoposide-induced senescence. We used the geNorm algorithm to identify the most stable genes across all studied conditions. Gene Ontology analysis found that the most stable genes belonged to the KEGG category of Genetic Information Processing. The optimal combination of housekeeping genes for ECFC senescence was found to include four ribosomal protein genes; RPL13, RPL31, RPL37, and RPL30. The RT-qPCR validation confirmed that normalization with our novel panel was more sensitive in identifying senescence markers compared to commonly used genes such as ACTB, UBC, and GAPDH.
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