ArticleScientific reports2019
Introgression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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17 citing papers in PubMed, 27 citations in OpenAlex.
- Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.Nature communications · 2026Article
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- Unravelling the role of mitochondrial DNA in hybrid incompatibility within species of the Anopheles gambiae complex.Scientific reports · 2024Article
- Cas9/guide RNA-based gene-drive dynamics following introduction and introgression into diverse anopheline mosquito genetic backgrounds.BMC genomics · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Y chromosome shredding in Anopheles gambiae: Insight into the cellular dynamics of a novel synthetic sex ratio distorter.PLoS genetics · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii.Insect molecular biology · 2023Article
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
- Article
- CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes.PLoS neglected tropical diseases · 2020Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Next-generation gene drive for population modification of the malaria vector mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evaluating the probability of CRISPR-based gene drive contaminating another species.Evolutionary applications · 2020Article
- Copy-number variation introduced by long transgenes compromises mouse male fertility independently of pachytene checkpoints.Chromosoma · 2020Article
- High-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
I-PpoI is a homing endonuclease that has a high cleavage activity and specificity for a conserved sequence within the ribosomal rDNA repeats, located in a single cluster on the Anopheles gambiae X chromosome. This property has been exploited to develop a synthetic sex ratio distortion system in this mosquito species. When I-PpoI is expressed from a transgene during spermatogenesis in mosquitoes, the paternal X chromosome is shredded and only Y chromosome-bearing sperm are viable, resulting in a male-biased sex ratio of >95% in the progeny. These distorter male mosquitoes can efficiently suppress caged wild-type populations, providing a powerful tool for vector control strategies. Given that malaria mosquito vectors belong to a species complex comprising at least two major vectors, we investigated whether the sex distorter I-PpoI, originally integrated in the A. gambiae genome, could be transferred via introgression to the sibling vector species Anopheles arabiensis. In compliance with Haldane's rule, F1 hybrid male sterility is known to occur in all intercrosses among members of the Anopheles gambiae complex. A scheme based on genetic crosses and transgene selection was used to bypass F1 hybrid male sterility and introgress the sex distorter I-PpoI into the A. arabiensis genetic background. Our data suggest that this sex distortion technique can be successfully applied to target A. arabiensis mosquitoes.
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