ArticleScientific reports2019
Liberibacter crescens biofilm formation in vitro: establishment of a model system for pathogenic 'Candidatus Liberibacter spp.'
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Galleria mellonella possesses the essential nutritional needs to host the fastidious Huanglongbing bacterial pathogen 'Candidatus Liberibacter asiaticus'.Communications biology · 2025Article
- Amicoumacins produced by the native citrus microbiome isolateApplied and environmental microbiology · 2025Article
- Article
- A serralysin-like protein ofFrontiers in microbiology · 2022Article
- Photodynamic control of citrus crop diseases.World journal of microbiology & biotechnology · 2021Review
- The Genome of "Frontiers in microbiology · 2021Article
- Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp.Nature communications · 2020Article
- Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions.Molecular plant · 2020Review
- Zinkicide Is a ZnO-Based Nanoformulation with Bactericidal Activity against Liberibacter crescens in Batch Cultures and in Microfluidic Chambers Simulating Plant Vascular Systems.Applied and environmental microbiology · 2020Article
- The flagella of 'Candidatus Liberibacter asiaticus' and its movement in planta.Molecular plant pathology · 2020Article
- Host-free biofilm culture of "Biofilm · 2019Article
- Challenging battles of plants with phloem-feeding insects and prokaryotic pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Growth Dynamics and Survival ofApplied and environmental microbiology · 2019Article
- Review
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A novel Zn chelate (TSOL) that moves systemically in citrus plants inhibits growth and biofilm formation of bacterial pathogens.PloS one · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Liberibacter genus comprises insect endosymbiont bacterial species that cause destructive plant diseases, including Huanglongbing in citrus and zebra chip in potato. To date, pathogenic 'Candidatus Liberibacter spp.' (CLs) remain uncultured, therefore the plant-associated Liberibacter crescens (Lcr), only cultured species of the genus, has been used as a biological model for in vitro studies. Biofilm formation by CLs has been observed on the outer midgut surface of insect vectors, but not in planta. However, the role of biofilm formation in the life cycle of these pathogens remains unclear. Here, a model system for studying CLs biofilms was developed using Lcr. By culture media modifications, bovine serum albumin (BSA) was identified as blocking initial cell-surface adhesion. Removal of BSA allowed for the first time observation of Lcr biofilms. After media optimization for biofilm formation, we demonstrated that Lcr attaches to surfaces, and form cell aggregates embedded in a polysaccharide matrix both in batch cultures and under flow conditions in microfluidic chambers. Biofilm structures may represent excellent adaptive advantages for CLs during insect vector colonization helping with host retention, immune system evasion, and transmission. Future studies using the Lcr model established here will help in the understanding of the biology of CLs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.