ArticleNature communications2019
Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 267 citations in OpenAlex.
- Reprogramming energy system for growth and bioproduction from CO₂ and methanol in Escherichia coli.Nature communications · 2026Article
- Electrostatic Engineering of Phosphoketolase Enhances Activity on Small Nonphosphorylated Sugars and Improves Cell-Free ATP Regeneration from Inexpensive C2-Substrates.The journal of physical chemistry. B · 2026Article
- Towards an enzyme cascade synthesis of the bulk chemical acrylic acid.Nature communications · 2026Article
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Plug-and-play - enzymatic amino acid production from methanol and carbon dioxide.Nature communications · 2026Article
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- Advances in synthetic biology for engineering methylotrophic microbial cell factories.Journal of bacteriology · 2026Review
- Photo-enzyme-membrane for ethylene glycol synthesis.Nature communications · 2026Article
- N-Heterocyclic Carbene (NHC) Catalysis in Enzymatic Organic Synthesis.Coordination chemistry reviews · 2026Article
- Engineering energy-efficient Saccharomyces cerevisiae for methanol and CONature communications · 2026Article
- Multicriteria evaluation of ethylene glycol assimilation pathways.Journal of industrial microbiology & biotechnology · 2026Article
- A blueprint for designing the next-generation of synthetic CNature communications · 2025Review
- Carbon sequestration pathways in microorganisms: Advances, strategies, and applications.Engineering microbiology · 2025Review
- Review
- Creating novel metabolic pathways by protein engineering for bioproduction.Trends in biotechnology · 2025Review
- Cell-Free Reaction System for ATP Regeneration from d-Fructose.ACS synthetic biology · 2025Article
- A Practical Guide to Computational Tools for Engineering Biocatalytic Properties.International journal of molecular sciences · 2025Review
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts.Nature communications · 2025Article
- The second wave of formose research.BBA advances · 2025Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
29 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetyl-CoA is a fundamental metabolite for all life on Earth, and is also a key starting point for the biosynthesis of a variety of industrial chemicals and natural products. Here we design and construct a Synthetic Acetyl-CoA (SACA) pathway by repurposing glycolaldehyde synthase and acetyl-phosphate synthase. First, we design and engineer glycolaldehyde synthase to improve catalytic activity more than 70-fold, to condense two molecules of formaldehyde into one glycolaldehyde. Second, we repurpose a phosphoketolase to convert glycolaldehyde into acetyl-phosphate. We demonstrated the feasibility of the SACA pathway in vitro, achieving a carbon yield ~50%, and confirmed the SACA pathway by
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.