Evidence map›Paper›PMID 30913987›Full record

ArticleTissue engineering. Part C, Methods2019

A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation.

Bai Yang, Cecillia Lui, Enoch Yeung, Hiroshi Matsushita, Anjana Jeyaram, Isaree Pitaktong, Takahiro Inoue, Zayneb Mohamed, Chin Siang Ong, Deborah DiSilvestre and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part C, Methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 26 citations in OpenAlex.

  1. Generation and applications of cardiac spheroids.npj biomedical innovations · 2025
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  12. Current challenges in three-dimensional bioprinting heart tissues for cardiac surgery.European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery · 2020
    Review
  13. Recent Development in Therapeutic Cardiac Patches.Frontiers in cardiovascular medicine · 2020
    Review
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Bai Yang1 Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
Cecillia Lui2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Enoch Yeung2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Hiroshi Matsushita2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Anjana Jeyaram3 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland.
Isaree Pitaktong2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Takahiro Inoue2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Zayneb Mohamed2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Chin Siang Ong2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Deborah DiSilvestre4 Division of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland.
Steven M Jay3 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland.
Leslie Tung5 Fischell Department of Bioengineering, University of Maryland, College Park, Maryland.
Gordon Tomaselli4 Division of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland.
Chunye Ma1 Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, Jilin, China.
Narutoshi Hibino2 Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland.
Johns Hopkins Hospital · USJohns Hopkins University · USJilin University · CNUniversity of Maryland, College Park · US

Funding

Controlling Exosome Noncoding RNA Cargo for Enhanced Wound HealingR01HL141611 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI JAY, STEVEN MICHAEL · 2018 to 2022
$1.9M
NHLBI NIH HHS R01 HL141611
6 · The paper itself

Abstract

Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore,

Indexed as

AnimalsArteriesBiocompatible MaterialsCell LineCell SizeCell SurvivalCollagenElectrocardiographyExosomesFemaleHeartHumansLigationRatsRats, Inbred LewRats, NudeBiocompatible MaterialsCollagencardiac tissue engineeringhanging drop spheroidsheart failuremold-based method

Identifiers

PMID30913987
PMCPMC13215059
OpenAlexW2925188367

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.