ArticlePloS one2019
Detection of Schistosoma mansoni-derived DNA in human urine samples by loop-mediated isothermal amplification (LAMP).
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 30 citations in OpenAlex.
- Test accuracy of loop-mediated isothermal amplification for schistosomiasis in low endemicity areas: a systematic review and meta-analysis.Infectious diseases of poverty · 2025Pooled it
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Novel Antischistosomal Drug Targets: Identification of Alkaloid Inhibitors of SmTGR via Integrated In Silico Methods.Pathogens (Basel, Switzerland) · 2025Article
- Molecular detection and identification of Trichobilharzia: development of a LAMP, qPCR, and multiplex PCR toolkit.Parasites & vectors · 2025Article
- Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection ofMicroorganisms · 2023Article
- Development of a Duplex LAMP Assay with Probe-Based Readout for Simultaneous Real-Time Detection ofInternational journal of molecular sciences · 2023Article
- Advances in Diagnosis of Schistosomiasis: Focus on Challenges and Future Approaches.International journal of general medicine · 2023Review
- Application of a low-cost, specific, and sensitive loop-mediated isothermal amplification (LAMP) assay to detect Plasmodium falciparum imported from Africa.Molecular and biochemical parasitology · 2022Article
- Development of real-time and lateral flow recombinase polymerase amplification assays for rapid detection ofFrontiers in microbiology · 2022Article
- Assessment of the accuracy of 11 different diagnostic tests for the detection ofFrontiers in microbiology · 2022Article
- A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil.Parasites & vectors · 2021Article
- Application of a Genus-Specific LAMP Assay for Schistosome Species to DetectJournal of clinical medicine · 2021Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania.Infectious diseases of poverty · 2021Article
- Loop-Mediated Isothermal Amplification in Schistosomiasis.Journal of clinical medicine · 2021Review
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment.Infectious diseases of poverty · 2020Article
- Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65.BMC infectious diseases · 2020Article
- Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSchistosoma mansoni is the main species causing hepatic and intestinal schistosomiasis in Sub-Saharan Africa, and it is the only species in South America. Adult stages of the parasite reside in the mesenteric venous plexus of infected hosts, and eggs are shed in feces. Collecting patient stool samples for S. mansoni diagnostic purposes is difficult in large-scale field trials. Urine samples would be an alternative approach for molecular S. mansoni detection since they have several advantages over stool samples, including better handling, management and storage. Additionally, loop-mediated isothermal amplification (LAMP) technology is a powerful molecular diagnostic tool for infectious diseases, particularly under field conditions in developing countries. The present study aimed to assess the effectiveness of our previously developed LAMP assay (SmMIT-LAMP) for S. mansoni-specific detection in clinical urine samples. METHODOLOGY/PRINCIPAL
findingsThe sensitivity of SmMIT-LAMP in urine was established in simulated fresh human urine samples artificially spiked with genomic DNA from S. mansoni. LAMP for 120 min instead of 60 min improved the sensitivity, reaching values of 0.01 fg/μL. A set of well-defined frozen stored human urine samples collected from Sub-Saharan immigrant patients was selected from a biobank to evaluate the diagnostic validity of SmMIT-LAMP. The set included urine samples from patients with microscopy-confirmed infections with S. mansoni, S. haematobium and other nonschistosome parasites, as well as urine samples from patients with microscopy-negative eosinophilia without a confirmed diagnosis. The SmMIT-LAMP was incubated for 60 and 120 min. A longer incubation time was shown to increase the LAMP-positive results in patient urine samples. We also tested urine samples from mice experimentally infected with S. mansoni, and LAMP-positive results were obtained from the third week after infection. A real-time LAMP assay was also performed with three individual urine samples. CONCLUSIONS/SIGNIFICANCE: The SmMIT-LAMP could effectively detect S. mansoni DNA in mouse urine samples and produced promising results for human clinical samples. The detection of S. mansoni DNA in mouse urine samples from the third week after infection indicates that early diagnosis of active S. mansoni infection is possible using urine as a source of DNA. Further studies are still needed, but our method could be used as a promising molecular tool applicable to urine samples to diagnose human intestinal schistosomiasis caused by S. mansoni.
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