Evidence map›Paper›PMID 30912976›Full record

ArticleDento maxillo facial radiology2019

Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations.

Niels Belmans, Liese Gilles, Piroska Virag, Mihaela Hedesiu, Benjamin Salmon, Sarah Baatout, Stéphane Lucas, Reinhilde Jacobs, Ivo Lambrichts, Marjan Moreels

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Dento maxillo facial radiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05532514 (EFFECT OF LIMITED VOLUME CONE BEAM COMPUTED TOMOGRAPHY ON MICRONUCLEI CELLS COUNT OF BUCCAL MUCOSA), which is not on this map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05532514 unknown statusnot on this mapstarted 2022, after this paper: background citation

Effect of limited volume cone beam computed tomography on micronuclei cells count of buccal mucosa

TypeobservationalSponsorPostgraduate Institute of Dental Sciences RohtakRan2022 to 2022Enrolled63ConditionsMicronuclei CountArmsMICRONUCLEI COUNT
3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 4 countries.

Niels BelmansMorphology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
Liese GillesMorphology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
Piroska Virag'Iuliu Hatieganu' University of Medicine and Pharmacy, Department of Oral and Maxillofacial Radiology, Cluj-Napoca, Romania.
Mihaela Hedesiu'Iuliu Hatieganu' University of Medicine and Pharmacy, Department of Oral and Maxillofacial Radiology, Cluj-Napoca, Romania.
Benjamin SalmonDepartment of Orofacial Pathologies, Imaging and Biotherapies Lab and Dental Medicine, Paris Descartes University - Sorbonne Paris Cité, Bretonneau Hospital, HUPNVS, AP-HP, Paris, France.
Sarah BaatoutBelgian Nuclear Research Centre, Radiobiology Unit, SCK•CEN, Mol, Belgium.
Stéphane LucasUniversity of Namur, Research Institute for Life Sciences, Namur, Belgium.
Reinhilde JacobsDepartment of Imaging and Pathology Katholieke Universiteit Leuven, OMFS IMPATH Research Group, and University Hospitals, Oral and Maxillofacial Surgery, Dentomaxillofacial Imaging Center, Leuven, Belgium.
Ivo LambrichtsMorphology Group, Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.
Marjan MoreelsBelgian Nuclear Research Centre, Radiobiology Unit, SCK•CEN, Mol, Belgium.
Hasselt University · BEBelgian Nuclear Research Centre · BEIuliu Hațieganu University of Medicine and Pharmacy · ROKarolinska Institutet · SESorbonne Paris Cité · FRUniversity of Namur · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesCone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT.

methodsDevelopment and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage.

resultsValidation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination.

conclusionThe DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

Indexed as

Cone-Beam Computed TomographyMouth MucosaSalivaSpiral Cone-Beam Computed TomographyFemaleHumansMaleX-RaysBuccal mucosal cellsDental cone-beam CTDNA Double strand breaksOxidative stressSaliva

Identifiers

PMID30912976
PMCPMC6747439
OpenAlexW2922707887

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.