ArticleCurrent genetics2019
MaPmt4, a protein O-mannosyltransferase, contributes to cell wall integrity, stress tolerance and virulence in Metarhizium acridum.
Article in Current genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Role of MicroRNA-Like RNAs in the Regulation of Spore Morphological Differences in the Entomopathogenic FungusPolish journal of microbiology · 2022Article
- Host-Pathogen Interactions betweenJournal of fungi (Basel, Switzerland) · 2022Review
- Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model HostMolecules (Basel, Switzerland) · 2021Article
- Structural, Evolutionary, and Functional Analysis of the Protein O-Mannosyltransferase Family in Pathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Tolerance to Abiotic Factors of Microsclerotia and Mycelial Pellets FromFrontiers in fungal biology · 2021Article
- Activation of cellular immune response in insect model host Galleria mellonella by fungal α-1,3-glucan.Pathogens and disease · 2020Article
- Phenotypic and molecular insights into heat tolerance of formulated cells as active ingredients of fungal insecticides.Applied microbiology and biotechnology · 2020Review
- The putative deubiquitinating enzyme MoUbp4 is required for infection-related morphogenesis and pathogenicity in the rice blast fungus Magnaporthe oryzae.Current genetics · 2020Article
- MaPacC, a pH-responsive transcription factor, negatively regulates thermotolerance and contributes to conidiation and virulence in Metarhizium acridum.Current genetics · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In eukaryotic cells, protein O-glycosylation is an essential protein modification. Analysis of the Metarhizium acridum genome database revealed a total of three O-glycoside mannosyltransferase homologs (Pmt1, Pmt2 and Pmt4), closely related to Saccharomyces cerevisiae Pmt1, Pmt2, and Pmt4. In this study, the functions of MaPmt4, encoding a protein O-mannosyltransferase in M. acridum, were characterized using disruption and complementation strategies. Disruption of MaPmt4 delayed the conidial germination and reduced the fungal tolerances to heat shock and UV-B irradiation, but did not affect conidial yield. Inactivation of MaPmt4 displayed increased sensitivity to cell wall-perturbing agents, formed thinner cell walls, and changed composition of fungal cell wall, demonstrating that MaPmt4 was also important to maintain fungal cell wall integrity. Bioassays by topical inoculation and intrahemocoel injection showed that the MaPmt4 deletion mutant exhibited greatly reduced virulence. The subsequent examination revealed that the inactivation of MaPmt4 impaired appressorium formation, decreased fungal growth in locust hemolymph in vitro, and boosted insect immune responses, the latter in part potentially owing to the changes in conidial surface structures, and thus attenuated the virulence of MaPmt4 deletion mutant. Furthermore, the results of comparative proteomics showed that MaPmt4 played important roles in fungal cell wall integrity, stress tolerances, and virulence via broad genetic pathways.
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