Evidence map›Paper›PMID 30911168›Full record

ArticleNature2019

Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH.

Chee-Huat Linus Eng, Michael Lawson, Qian Zhu, Ruben Dries, Noushin Koulena, Yodai Takei, Jina Yun, Christopher Cronin, Christoph Karp, Guo-Cheng Yuan and 1 more

Abstract read
In one paragraph

Article in Nature, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1,139 papers.

0numbers the graph read from it
0cells of the map it votes in
1,139citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1,139 citing papers in PubMed.

  1. Immunophenotyping by Spatial Biology.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Spatial Transcriptomics Analysis of Ewing Sarcoma.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Insights Into Spatial Transcriptomics: Exploring Recent Technical Developments and Their Diverse Applications.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Article
  20. Review

1,079 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Chee-Huat Linus EngDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.
Michael LawsonDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Qian ZhuDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard T.H.Chan School of Public Health, Boston, MA, USA.
Ruben DriesDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard T.H.Chan School of Public Health, Boston, MA, USA.
Noushin KoulenaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Yodai TakeiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Jina YunDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Christopher CroninDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Christoph KarpDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Guo-Cheng YuanDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard T.H.Chan School of Public Health, Boston, MA, USA.
Long CaiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. lcai@caltech.edu.

Funding

Validating Theoretical Models with Neurophysiology and OptogeneticsU19NS107613 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI ADESNIK, HILLEL · 2018 to 2022
$16.7M
MEMOIR: Recording, and in situ readout of cell lineage and transcriptional historyR01MH116508 · NIMH · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, ELOWITZ, MICHAEL B · 2017 to 2021
$6.9M
A Spatially Resolved Molecular Atlas of Human EndotheliumU54HL145611 · NHLBI · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG · 2018 to 2021
$4.9M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01DA047732 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2018 to 2019
$1.6M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01EB021240 · NIBIB · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2015 to 2017
$1.4M
In situ transcriptome profiling in single cellsUG3HL145609 · NHLBI · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, YUAN, GUO-CHENG · 2018 to 2019
$750k
NHLBI NIH HHS U54 HL145611NHLBI NIH HHS UG3 HL145609NIBIB NIH HHS U01 EB021240NIDA NIH HHS U01 DA047732NIH HHS TR01 OD024686NIMH NIH HHS R01 MH116508NINDS NIH HHS U19 NS107613
6 · The paper itself

Abstract

Imaging the transcriptome in situ with high accuracy has been a major challenge in single-cell biology, which is particularly hindered by the limits of optical resolution and the density of transcripts in single cells

Indexed as

3T3 CellsAnimalsBrainDopaminergic NeuronsEndothelial CellsFemaleGene Expression ProfilingIn Situ Hybridization, FluorescenceLigandsMaleMiceMicrogliaOrgan SpecificityRNA, MessengerSingle-Cell AnalysisTranscriptomeLigandsRNA, Messenger

Identifiers

PMID30911168
PMCPMC6544023

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.