ArticleScientific reports2019
Molecular quantification of Plasmodium parasite density from the blood retained in used RDTs.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum.Cell reports methods · 2026Article
- Short report: surveillance of hrp2/hrp3 deletions in Plasmodium falciparum within Haiti, 2021-2023, using RDTs.Malaria journal · 2026Article
- Rapid diagnostic test kit as a source of DNA in comparison with filter paper for malaria molecular surveillance and drug resistance monitoring: a cross-sectional study.Malaria journal · 2026Article
- A single dose of azithromycin versus placebo for malaria in infants: A cluster-randomized trial.PLOS global public health · 2026Article
- Automated total nucleic acid extraction with magnetic beads for the detection of Plasmodium falciparum in large study cohorts.Malaria journal · 2024Article
- Treatment Failure in a UK Malaria Patient Harboring Genetically Variant Plasmodium falciparum From Uganda With Reduced In Vitro Susceptibility to Artemisinin and Lumefantrine.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024Article
- Parasite clearance dynamics in children hospitalised with severe malaria in the Ho Teaching Hospital, Volta Region, Ghana.Parasite epidemiology and control · 2022Article
- Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania.Scientific reports · 2022Article
- Analysis of nucleic acids extracted from rapid diagnostic tests reveals a significant proportion of false positive test results associated with recent malaria treatment.Malaria journal · 2022Article
- Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda.Malaria journal · 2022Article
- Persistent Submicroscopic Plasmodium falciparum Parasitemia 72 Hours after Treatment with Artemether-Lumefantrine Predicts 42-Day Treatment Failure in Mali and Burkina Faso.Antimicrobial agents and chemotherapy · 2021Article
- Failure of rapid diagnostic tests in Plasmodium falciparum malaria cases among travelers to the UK and Ireland: Identification and characterisation of the parasites.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2021Article
- Community-based molecular and serological surveillance of subclinical malaria in Myanmar.BMC medicine · 2021Article
- Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.Scientific reports · 2020Article
- Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification.Malaria journal · 2020Article
- A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections.EBioMedicine · 2020Article
- Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal.Malaria journal · 2020Article
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11 authors.
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Abstract
Most malaria-endemic countries are heavily reliant upon rapid diagnostic tests (RDT) for malaria case identification and treatment. RDT previously used for malaria diagnosis can subsequently be used for molecular assays, including qualitative assessment of parasite species present or the carriage of resistance markers, because parasite DNA can be extracted from the blood inside the RDT which remains preserved on the internal components. However, the quantification of parasite density has not previously been possible from used RDT. In this study, blood samples were collected from school-age children in Western Kenya, in the form of both dried blood spots on Whatman filter paper, and the blood spot that is dropped into rapid diagnostic tests during use. Having first validated a robotic DNA extraction method, the parasite density was determined from both types of sample by duplex qPCR, and across a range of densities. The methods showed good agreement. The preservation of both parasite and human DNA on the nitrocellulose membrane inside the RDT was stable even after more than one year's storage. This presents a useful opportunity for researchers or clinicians wishing to gain greater information about the parasite populations that are being studied, without significant investment of resources.
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