Evidence map›Paper›PMID 30910463›Full record

ArticleInternational journal for parasitology2019

Intracellular iron availability modulates the requirement for Leishmania Iron Regulator 1 (LIR1) during macrophage infections.

Amrita Sarkar, Norma W Andrews, Maria Fernanda Laranjeira-Silva

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal for parasitology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Evaluation of Beauvericin's activity and mode of action against all life stages ofFrontiers in cellular and infection microbiology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Amrita SarkarDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA.
Norma W AndrewsDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA.
Maria Fernanda Laranjeira-SilvaDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA; Department of Animal and Avian Sciences, University of Maryland, College Park, MD, USA; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, SP, Brazil. Electronic address: mfernandals@usp.br.
University of Maryland, College Park · USUniversidade de São Paulo · BR

Funding

Molecular Determinants of Intracellular Survival and Replication in LeishmaniaR01AI067979 · NIAID · YALE UNIVERSITY · PI ANDREWS, NORMA WINDSOR · 2007 to 2018
$3.8M
NIAID NIH HHS R01 AI067979
6 · The paper itself

Abstract

The Leishmania plasma membrane transporter Leishmania Iron Regulator 1 (LIR1) facilitates iron export and is required for parasite virulence. By modulating macrophage iron content, we investigated the host site where LIR1 regulates Leishmania amazonensis infectivity. In bone marrow-derived macrophages, LIR1 null mutants demonstrated a paradoxical increase in virulence during infections in heme-depleted media, while wild-type growth was inhibited under the same conditions. Loading the endocytic pathway of macrophages with cationized ferritin prior to infection reversed the effect of heme depletion on both strains. Thus, LIR1 contributes to Leishmania virulence by protecting the parasites from toxicity resulting from iron accumulation inside parasitophorous vacuoles.

Indexed as

AnimalsFerritinsGene Knockout TechniquesIronIron DeficienciesIron Regulatory Protein 1LeishmaniaMacrophagesMiceMice, Inbred C57BLMicroscopy, ConfocalVirulenceFerritinsIronIron Regulatory Protein 1AmastigotesCationic ferritinDrug targetEndocytic pathwayHemeVirulence

Identifiers

PMID30910463
PMCPMC6527483
OpenAlexW2923646916

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.