Evidence map›Paper›PMID 30910020›Full record

ArticleMethods in enzymology2019

Assays for ubiquitin-like protein ligation and proteasome function in archaea.

Xian Fu, Zachary Adams, Julie Maupin-Furlow

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Xian FuDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Zachary AdamsDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Julie Maupin-FurlowDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States; University of Florida, Genetics Institute, Gainesville, FL, United States. Electronic address: jmaupin@ufl.edu.
University of Florida · US

Funding

Ubiquitin-like Small Archaeal Protein Modification (SAMPylation)R01GM057498 · NIGMS · UNIVERSITY OF FLORIDA · PI MAUPIN-FURLOW, JULIE A · 2000 to 2024
$4.9M
NIGMS NIH HHS R01 GM057498
6 · The paper itself

Abstract

Ubiquitin-like protein (Ubl) ligation is common to diverse archaea and targets many cellular pathways, including those associated with sulfur mobilization, and also tags proteins as substrates for degradation by the proteasome. Here we highlight protocols to assay proteasome function and Ubl ligation in archaea. A chase assay is described to monitor the impact of proteasome function on the stability of Ubl-modified proteins in the cell. A method to reconstitute Ubl ligation using a purified E1-like enzyme (UbaA), Ubl (SAMP2), methionine sulfoxide reductase A (MsrA), and cell lysate of an ΔmsrA ΔubaA Δsamp1-3 mutant is also described. MsrA is found to have the surprising ability to stimulate the formation of Ubl bonds. Haloferax volcanii, a halophilic archaeon originally isolated from the Dead Sea, serves as the model organism for these protocols.

Indexed as

Archaeal ProteinsEnzyme AssaysHaloferax volcaniiImmunoblottingProteasome Endopeptidase ComplexUbiquitinsArchaeal ProteinsProteasome Endopeptidase ComplexUbiquitinsArchaeaE1 enzymeJAMM/MPN+ proteaseMethionine sulfoxide reductaseMsrAProteasomeUbiquitinUbiquitin-like

Identifiers

PMID30910020
PMCPMC7301888
OpenAlexW2912956582

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.