ArticleParasites & vectors2019
Leishmania infantum-specific IFN-γ production in stimulated blood from cats living in areas where canine leishmaniosis is endemic.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 37 citations in OpenAlex.
- LeishVet update and current recommendations on feline leishmaniosis.Parasites & vectors · 2026Review
- Diagnostic potential of a multi-antigen ELISA for feline leishmaniosis.Parasites & vectors · 2026Article
- Detection of circulating immune complexes in feline leishmaniosis: first evidence and diagnostic implications.Veterinary research communications · 2026Article
- Cytokine gene expression in feline leishmaniasis: why cats might be less clinically affected than dogs.Parasites & vectors · 2026Article
- Serosurveillance of Leishmania infantum in Zoo-Kept Animals in Spain.Zoonoses and public health · 2026Article
- Morphological aspects of the skin in felines with leishmaniasis: a histopathological study.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026Article
- IL-12 and IFN-γ in Response to Leishmania Infantum Antigens in Felines From an Endemic Area for Visceral Leishmaniasis.Parasite immunology · 2025Article
- First report of a cat (Felis catus) infected by Leishmania (Viannia) guyanensis from Southeastern Brazil.Veterinary research communications · 2025Article
- Assessment of the exposure toThe veterinary quarterly · 2024Article
- The erythrocyte sedimentation rate and other markers of inflammation in cats tested for Leishmania infantum and feline immunodeficiency virus antibodies.Parasites & vectors · 2024Article
- Article
- A cross-sectional serosurvey of SARS-CoV-2 and co-infections in stray cats from the second wave to the sixth wave of COVID-19 outbreaks in Spain.Veterinary research communications · 2023Article
- A Case of Feline Leishmaniosis with Panniculitis.Case reports in veterinary medicine · 2023Article
- Hemogram Findings in Cats from an Area Endemic forVeterinary sciences · 2022Article
- Article
- Review and statistical analysis of clinical management of feline leishmaniosis caused by Leishmania infantum.Parasites & vectors · 2022Review
- Canine Cytokines Profile in an Endemic Region ofVeterinary sciences · 2022Article
- Association between feline immunodeficiency virus and Leishmania infantum infections in cats: a retrospective matched case-control study.Parasites & vectors · 2022Article
- Leishmaniosis caused byVeterinary and animal science · 2022Article
- Epidemiological and diagnostic aspects of feline leishmaniasis with emphasis on Brazil: a narrative review.Parasitology research · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFeline leishmaniosis caused by Leishmania infantum is considered a rare disease in endemic areas, whereas subclinical infections are common. Immune response plays a key role in driving the course of L. infantum infection in other host species; however, the feline cell-mediated immune response to L. infantum infection has not yet been investigated. The aim of this study was to determine the cell-mediated immune response specific to L. infantum by means of interferon (IFN)-γ release in whole blood assay from cats living in endemic areas (66 in Sicily and 113 in Catalonia) and to compare with antibody levels to L. infantum [enzyme-linked immunosorbent assay (ELISA) and immunofluorescence antibody test (IFAT)], blood parasite load and retroviral infections.
resultsMost cats (n = 140) were L. infantum antibody negative and only 22% (n = 39) were positive. Only 9 and 2% of tested cats had a feline immunodeficency virus (FIV) infection or a feline leukemia virus (FeLV) infection, respectively. Thirty-two cats out of 179 (18%) produced IFN-γ after stimulation with L. infantum soluble antigen (LSA) while the majority of cats (93%) produced IFN-γ after stimulation with concanavalin A (ConA). Six LSA-IFN-γ-producer cats were seropositive (three to ELISA and five to IFAT) but they were polymerase chain reaction (PCR) negative, while only one cat was antibody- and PCR-positive. Significant positive correlations were found between IFN-γ concentrations after stimulation with LSA and ConA, and between serology and PCR testing. No association was found between FIV status and LSA or ConA-IFN-γ production. Combining PCR, serology and specific IFN-γ concentration results, we found that 36% of cats studied were exposed to L. infantum.
conclusionsAs expected, cats from endemic areas produce IFN-γ after ex vivo blood stimulation with LSA and therefore are able to activate a cell-mediated adaptive immune response against the parasite that is variably associated with antibody or blood PCR positivity. The association of this assay to serological and molecular tests provides a better estimate of cat exposure to L. infantum.
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