ArticleMicrobial cell factories2019
Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 61 citations in OpenAlex.
- One-site polarity switch enhances catalytic efficiency and stability of D-allulose 3-epimerase via flexibility-rigidity rebalancing.Scientific reports · 2026Article
- Comprehensive Analysis of Allulose Production: A Review and Update.Foods (Basel, Switzerland) · 2024Review
- Biochemical characterization, structure-guided mutagenesis, and application of a recombinant D-allulose 3-epimerase fromFrontiers in bioengineering and biotechnology · 2024Article
- Non-Native Site-Selective Enzyme Catalysis.Chemical reviews · 2023Review
- Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose.Frontiers in microbiology · 2022Review
- D-Allulose (D-Psicose) Biotransformation From Allitol by a Newly Found NAD(P)-Dependent Alcohol Dehydrogenase FromFrontiers in microbiology · 2022Article
- Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021Review
- Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.FEBS open bio · 2021Article
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis.Applied and environmental microbiology · 2020Article
- Biocatalytic Synthesis of D-Allulose Using Novel D-Tagatose 3-Epimerase FromFrontiers in chemistry · 2020Article
- Expression, Purification, Refolding, and Characterization of aFrontiers in bioengineering and biotechnology · 2020Article
- Review onFrontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundA novel D-allulose 3-epimerase from Staphylococcus aureus (SaDAE) has been screened as a D-allulose 3-epimerase family enzyme based on its high specificity for D-allulose. It usually converts both D-fructose and D-tagatose to respectively D-allulose and D-sorbose. We targeted potential biocatalysts for the large-scale industrial production of rare sugars.
resultsSaDAE showed a high activity on D-allulose with an affinity of 41.5 mM and catalytic efficiency of 1.1 s
conclusionsWe redesigned SaDAE for thermostability and biocatalytic production of D-allulose. The research will aid the development of industrial biocatalysts for D-allulose.
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