ArticlePloS one2019
The effect of the staining technique on morphological and morphometric parameters of boar sperm.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 44 citations in OpenAlex.
- Comparative Evaluation of Staining Techniques in Thawed Cryopreserved Dog Semen.Veterinary sciences · 2026Article
- Utilization of artificial intelligence in Men's Health: Opportunities for innovation and quality improvement.International journal of impotence research · 2026Review
- Design and Implementation of a Deep Learning System to Analyze Bovine Sperm Morphology.Veterinary sciences · 2025Article
- Comparative Assessment of Sperm Morphology in Liquid-Preserved Boar Semen Using Cytological Stains.Animals : an open access journal from MDPI · 2025Article
- Effect of Pre-Freezing 18 °C Holding Time on Post-Thaw Motility and Morphometry of Cryopreserved Boar Epididymal Sperm.Animals : an open access journal from MDPI · 2025Article
- Assessment of Boar Semen Morphology and Morphometry.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing oxido-inflammation and apoptotic mediators.International journal of biochemistry and molecular biology · 2024Article
- Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls.Animals : an open access journal from MDPI · 2023Article
- Multi- and transcriptomic changes of chronic exposure to bisphenol A reveals reproductive toxicity in male zebrafish.Fish physiology and biochemistry · 2023Article
- Effects of six kinds of sperm staining methods on human sperm size and evaluation of their staining effects.Journal of clinical laboratory analysis · 2022Article
- Morphometry of Boar Spermatozoa in Semen Stored at 17 °C-The Influence of the Staining Technique.Animals : an open access journal from MDPI · 2022Article
- Comparison of two staining techniques on the manual and automated canine sperm morphology analysis.Reproduction in domestic animals = Zuchthygiene · 2022Article
- Machine learning for sperm selection.Nature reviews. Urology · 2021Review
- Assessment of the Morphometry of Heads of Normal Sperm and Sperm with the Dag Defect in the Semen of Duroc Boars.Journal of veterinary research · 2021Article
- Evaluation of the Morphometry of Sperm from the Epididymides of Dogs Using Different Staining Methods.Animals : an open access journal from MDPI · 2021Article
- Spermatozoon head size - the main differentiating feature between spermatozoa of blue and white Arctic fox (Vulpes lagopus).Animal reproduction · 2021Article
- Morphometric Characteristics of the Spermatozoa of Blue Fox (Animals : an open access journal from MDPI · 2020Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm morphology and morphometry are important parameters in predicting fertility. Sperm are considered to be normal if the shape and size of the head, midpiece and tail fall within the classification for a given species. It is important to select the appropriate technique for staining the semen of a given species, because, as many authors have pointed out, some methods work well for one species but are not suitable for analysing another. The aim of the study was to assess the morphometric parameters of boar sperm following the use of different staining techniques and to verify the hypothesis that the staining technique affects the morphometric parameters of sperm. The staining method was found to significantly affect the dimensions of the boar sperm head. The semen stained by the SpermBlue technique had the closest morphometric sperm head parameters to those of the unstained sperm, so this technique, rather than the routinely used eosin and gentian complex, should be the leading technique in the evaluation of boar sperm morphometry. Silver nitrate staining reveals the structure of the sperm in the most detail; this method can be considered universal, and can be used independently or to supplement routine diagnostics. As the staining technique should interfere as little as possible with the structure of the sperm, while revealing its morphology in as much detail as possible, it is crucial to establish the natural dimensions of the unstained sperm head before determining the optimal technique and its reference values. The recommended or most commonly-used techniques are not always the best options for the staining and analysis of sperm of a given species.
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