Evidence map›Paper›PMID 30906572›Full record

ArticleConvergent science physical oncology2018

Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry.

Erik Gerdtsson, Milind Pore, Jana-Aletta Thiele, Anna Sandström Gerdtsson, Paymaneh D Malihi, Rafael Nevarez, Anand Kolatkar, Carmen Ruiz Velasco, Sophia Wix, Mohan Singh and 6 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Convergent science physical oncology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
3.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 75 citations in OpenAlex.

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  11. Liquid biopsy to personalize treatment for metastatic prostate cancer.American journal of translational research · 2024
    Review
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  18. Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy.International journal of molecular sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Erik GerdtssonBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Milind PoreBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Jana-Aletta ThieleBiomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, alej Svobody 76, 323 00 Pilsen, Czech Republic; Jana.A.Thiele@lfp.cuni.cz.
Anna Sandström GerdtssonBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Paymaneh D MalihiBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Rafael NevarezBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Anand KolatkarBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Carmen Ruiz VelascoBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Sophia WixBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Mohan SinghDivision of Hematology, Department of Medicine, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA; mohansin@usc.edu; akilmerc@usc.edu.
Anders CarlssonBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Amado J ZuritaDepartment of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 207, Houston, Texas; azurita@mdanderson.org; clogothe@mdanderson.org.
Christopher LogothetisDepartment of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 207, Houston, Texas; azurita@mdanderson.org; clogothe@mdanderson.org.
Akil A MerchantDivision of Hematology, Department of Medicine, USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA; mohansin@usc.edu; akilmerc@usc.edu.
James HicksBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
Peter KuhnBridge@USC, USC David and Dana Dornsife College of Letters, Arts, and Sciences, 3430 S Vermont Ave, TRF 114, MC3303, Los Angeles, CA, 90089-3303; gerdtsso@usc.edu; pore@usc.edu; agerdtss@usc.edu; pmalihi@usc.edu; rnevarez@usc.edu; kolatkar@usc.edu; ruizvela@usc.edu; swix@usc.edu; ncarlsso@usc.edu; jameshic@usc.edu; pkuhn@usc.edu.
LAC+USC Medical Center · USUniversity of Southern California · USThe University of Texas MD Anderson Cancer Center · USCharles University · CZ

Funding

CCR NIH HHS HHSN261200800001CNCI NIH HHS HHSN261200800001E
6 · The paper itself

Abstract

Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation of prognosis and treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods for molecular analysis of these rare cells must be developed and validated. Here, we describe the integration of imaging mass cytometry (IMC) using metal-labeled antibodies as implemented on the Fluidigm Hyperion Imaging System into the workflow of the previously established High Definition Single Cell Analysis (HD-SCA) assay for liquid biopsies, along with methods for image analysis and signal normalization. Using liquid biopsies from a metastatic prostate cancer case, we demonstrate that IMC can extend the reach of CTC characterization to include dozens of protein biomarkers, with the potential to understand a range of biological properties that could affect therapeutic response, metastasis and immune surveillance when coupled with simultaneous phenotyping of thousands of leukocytes.

Identifiers

PMID30906572
PMCPMC6430142
OpenAlexW2775045801

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.