Evidence map›Paper›PMID 30905884›Full record

ArticleThe Journal of reproduction and development2019

Mild hypothermia promotes the viability of in vitro-produced bovine blastocysts and their transcriptional expression of the cold-inducible transcription factor Rbm3 during in vitro culture.

Toshimichi Ishii, Koga Kawano, Nobumasa Tanaka, Kensuke Tomita, Naohiko Saito, Masayasu Yamada

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Toshimichi IshiiDepartment of Livestock Improvement and Reproduction, Mie Prefectural Livestock Research Center, Matsusaka 515-2324, Japan.
Koga KawanoLaboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Nobumasa TanakaDepartment of Livestock Improvement and Reproduction, Mie Prefectural Livestock Research Center, Matsusaka 515-2324, Japan.
Kensuke TomitaDepartment of Livestock Improvement and Reproduction, Mie Prefectural Livestock Research Center, Matsusaka 515-2324, Japan.
Naohiko SaitoDepartment of Livestock Improvement and Reproduction, Mie Prefectural Livestock Research Center, Matsusaka 515-2324, Japan.
Masayasu YamadaLaboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Kyoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we evaluated the effects of holding in vitro-produced bovine blastocysts under mild hypothermia (33°C or 35°C), by examining viability and hatching rates of day 7 blastocysts (day 0: in vitro fertilization) cultured for 6 days and transcriptional expression of cold-inducible transcription factors Cirp and Rbm3, implicated in mild hypothermia-induced cellular protection against various types of stress. In the normothermic control (38.5°C), viability of the embryos decreased rapidly after day 10, and most samples were degenerated on day 13. However, mild hypothermia, particularly at 33°C, resulted in maintenance of high embryonic survival rates until day 13 (77.1% on day 13) and significant increases in transcriptional expression of Rbm3 in day 11 embryos compared with those at 38.5°C. Thus, our results suggested that upregulation of Rbm3 may occur in response to mild hypothermia in many bovine embryos, providing insights into the effects of mild hypothermia on embryo quality.

Indexed as

Embryo Culture TechniquesGene Expression RegulationAnimalsBlastocystCattleCold TemperatureFemaleFertilization in VitroOvaryRNA-Binding ProteinsTime FactorsTranscriptional ActivationTranscription FactorsTranscription, GeneticRNA-Binding ProteinsTranscription FactorsBovine blastocystCirpEmbryo holdingMild-hypothermiaRbm3

Identifiers

PMID30905884
PMCPMC6584183
OpenAlexW2945846269

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.