Evidence map›Paper›PMID 30905759›Full record

ArticleInsect biochemistry and molecular biology2019

The three-dimensional structure and recognition mechanism of Manduca sexta peptidoglycan recognition protein-1.

Yingxia Hu, Xiaolong Cao, Xiuru Li, Yang Wang, Geert-Jan Boons, Junpeng Deng, Haobo Jiang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Yingxia HuDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA; Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
Xiaolong CaoDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA; Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
Xiuru LiComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Yang WangDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Geert-Jan BoonsComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA; Department of Chemistry, University of Georgia, Athens, GA, 30602, USA; Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CG, Utrecht, the Netherlands.
Junpeng DengDepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA. Electronic address: Junpeng.deng@okstate.edu.
Haobo JiangDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA. Electronic address: haobo.jiang@okstate.edu.
Oklahoma State University · USUniversity of Georgia · US

Funding

Initiation and Regulation of Prophenoloxidase ActivationR01GM058634 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI JIANG, HAOBO · 1999 to 2022
$4.0M
Activation and catalytic mechanisms of mosquito phenoloxidasesR21AI139998 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI JIANG, HAOBO · 2018 to 2019
$402k
Prophenoloxidases of Anopheles gambiaeR21AI112662 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI JIANG, HAOBO · 2014 to 2015
$381k
NIAID NIH HHS R21 AI112662NIAID NIH HHS R21 AI139998NIGMS NIH HHS R01 GM058634
6 · The paper itself

Abstract

Peptidoglycan recognition proteins (PGRPs) recognize bacteria through their unique cell wall constituent, peptidoglycans (PGs). PGRPs are conserved from insects to mammals and all function in antibacterial defense. In the tobacco hornworm Manduca sexta, PGRP1 and microbe binding protein (MBP) interact with PGs and hemolymph protease-14 precursor (proHP14) to yield active HP14. HP14 triggers a serine protease network that produces active phenoloxidase (PO), Spätzle, and other cytokines to stimulate immune responses. PGRP1 binds preferentially to diaminopimelic acid (DAP)-PGs of Gram-negative bacteria and Gram-positive Bacillus and Clostridium species than Lys-PGs of other Gram-positive bacteria. In this study, we synthesized DAP- and Lys-muramyl pentapeptide (MPP) and monitored their associations with M. sexta PGRP1 by surface plasmon resonance. The K

Indexed as

AnimalsCarrier ProteinsInsect ProteinsManducaMolecular Docking SimulationPeptidoglycanProtein Structure, SecondaryReceptors, Pattern RecognitionSurface Plasmon ResonanceCarrier ProteinsInsect ProteinsPeptidoglycanpeptidoglycan recognition proteinReceptors, Pattern RecognitionHemolymph proteinInsect immunityMelanizationPattern recognitionProphenoloxidase activationSerine protease

Identifiers

PMID30905759
PMCPMC7032066
OpenAlexW2922876419

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.