Evidence map›Paper›PMID 30905322›Full record

ReviewInfectious diseases of poverty2019

Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.

Miao-Han Deng, Lan-Yi Zhong, Okanurak Kamolnetr, Yanin Limpanont, Zhi-Yue Lv

Open access · goldAbstract readReview
In one paragraph

Review in Infectious diseases of poverty, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 3 pooled it
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 3 syntheses or guidelines pooled it, 58 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Review
  10. A loop-mediated isothermal amplification (LAMP) assay to identify isotype 1 β-tubulin locus SNPs in synthetic double-strandedJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022
    Article
  11. Article
  12. A review of testing and assurance methods forFood and waterborne parasitology · 2021
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. An accurate, rapid and simple loop-mediated isothermal amplification method forVeterinary research forum : an international quarterly journal · 2021
    Article
  18. Diagnostic Techniques for Soil-Transmitted Helminths - Recent Advances.Research and reports in tropical medicine · 2021
    Review
  19. Epidemiological, Morphological, and Morphometric Study onFrontiers in veterinary science · 2021
    Article
  20. Development of a New LAMP Assay for the Detection ofFrontiers in veterinary science · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Miao-Han DengZhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Lan-Yi ZhongZhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Okanurak KamolnetrFaculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Yanin LimpanontFaculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand. yanin.lim@mahidol.ac.th.
Zhi-Yue LvZhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. lvzhiyue@mail.sysu.edu.cn.
Mahidol Oxford Tropical Medicine Research Unit · THSun Yat-sen University · CNFifth Affiliated Hospital of Sun Yat-sen University · CN

Funding

the National Key Research and Development Program of China 2016YFC1202003
6 · The paper itself

Abstract

backgroundHelminths are endemic in more than half of the world's countries, raising serious public health concerns. Accurate diagnosis of helminth infection is crucial to control strategies. Traditional parasitological methods, serological tests and PCR-based assays are the major means of the diagnosis of helminth infection, but they are time-consuming and/or expensive, and sometimes provide inaccurate results. Loop mediated isothermal amplification (LAMP) assay, a sensitive, simple and rapid method was therefore developed for detection of helminths. This study aims to discuss the current status of application of LAMP on helminths detection and to make a comprehensive evaluation about this updated technology and its future outlook by comparing with several other diagnostic methods. MAIN BODY: This review summarizes LAMP assay applied for helminth detection and helminthiasis surveillance. The basic principle of LAMP is introduced to help better understand its characteristics and each reported assay is assessed mainly based on its detection sensitivity, specificity and limitations, in comparison with other common diagnostic tests. Moreover, we discuss the limitations of the assays so as to clarify some potential ways of improvement.

conclusionsHere, we summarize and discuss the advantages, disadvantages and promising future of LAMP in heliminth detection, which is expected to help update current knowledge and future perspectives of LAMP in highly sensitive and specific diagnosis and surveillance of helminthiasis and other parasitic diseases, and can contribute to the elimination of the diseases from endemic areas.

Indexed as

AnimalsDNA, HelminthFood MicrobiologyHelminthiasisHelminthsHumansNucleic Acid Amplification TechniquesPolymerase Chain ReactionSensitivity and SpecificityDNA, HelminthEpidemiological surveillanceField surveyHelminthLoop-mediated isothermal amplificationPoint-of-care-test

Identifiers

PMID30905322
PMCPMC6432754
OpenAlexW2943388774

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.