ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2019
Competition between mobile genetic elements drives optimization of a phage-encoded CRISPR-Cas system: insights from a natural arms race.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- Bacterial immune systems as causes and consequences of microbiome structure.PLoS biology · 2025Article
- Cas1 mediates the interference stage in a phage-encoded CRISPR-Cas system.Nature chemical biology · 2024Article
- A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread.Nucleic acids research · 2024Article
- AJournal of bacteriology · 2024Article
- Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate ofmSphere · 2024Article
- A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread.bioRxiv : the preprint server for biology · 2024Article
- AbioRxiv : the preprint server for biology · 2024Article
- March of the proviruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- AeLife · 2024Article
- Demographic fluctuations in bloodstreamnpj antimicrobials and resistance · 2024Article
- AbioRxiv : the preprint server for biology · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Mechanistic insights into DNA binding and cleavage by a compact type I-F CRISPR-Cas system in bacteriophage.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Role of Bacteriophages in the Evolution of Pathogenic Vibrios and Lessons for Phage Therapy.Advances in experimental medicine and biology · 2023Article
- A phage weaponizes a satellite recombinase to subvert viral restriction.Nucleic acids research · 2022Article
- A phage parasite deploys a nicking nuclease effector to inhibit viral host replication.Nucleic acids research · 2022Article
- The Vibrio cholerae Seventh Pandemic Islands act in tandem to defend against a circulating phage.PLoS genetics · 2022Article
- CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids.Nucleic acids research · 2022Article
- Mechanisms and clinical importance of bacteriophage resistance.FEMS microbiology reviews · 2022Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
CRISPR-Cas systems function as adaptive immune systems by acquiring nucleotide sequences called spacers that mediate sequence-specific defence against competitors. Uniquely, the phage ICP1 encodes a Type I-F CRISPR-Cas system that is deployed to target and overcome PLE, a mobile genetic element with anti-phage activity in Vibrio cholerae. Here, we exploit the arms race between ICP1 and PLE to examine spacer acquisition and interference under laboratory conditions to reconcile findings from wild populations. Natural ICP1 isolates encode multiple spacers directed against PLE, but we find that single spacers do not interfere equally with PLE mobilization. High-throughput sequencing to assay spacer acquisition reveals that ICP1 can also acquire spacers that target the V. cholerae chromosome. We find that targeting the V. cholerae chromosome proximal to PLE is sufficient to block PLE and is dependent on Cas2-3 helicase activity. We propose a model in which indirect chromosomal spacers are able to circumvent PLE by Cas2-3-mediated processive degradation of the V. cholerae chromosome before PLE mobilization. Generally, laboratory-acquired spacers are much more diverse than the subset of spacers maintained by ICP1 in nature, showing how evolutionary pressures can constrain CRISPR-Cas targeting in ways that are often not appreciated through in vitro analyses. This article is part of a discussion meeting issue 'The ecology and evolution of prokaryotic CRISPR-Cas adaptive immune systems'.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.