ArticleJournal of bioscience and bioengineering2019
Ultrasonically-guided flow focusing generates precise emulsion droplets for high-throughput single cell analyses.
Article in Journal of bioscience and bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Potent Monoclonal Antibodies Against Circulating Yellow Fever Virus Strains from Donors Immunized with the 17D Vaccine.The American journal of tropical medicine and hygiene · 2026Article
- [Progress and prospect of separation and analysis of single-cell and single-particle exosomes].Se pu = Chinese journal of chromatography · 2025Review
- Beyond Single Clones: High-Throughput Sequencing in Antibody Discovery.Cold Spring Harbor protocols · 2025Article
- Large-scale antibody immune response mapping of splenic B cells and bone marrow plasma cells in a transgenic mouse model.Frontiers in immunology · 2023Article
- Immortalization and functional screening of natively paired human T cell receptor repertoires.Protein engineering, design & selection : PEDS · 2022Article
- Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron.PloS one · 2022Article
- Quality Control: Chain Pairing Precision and Monitoring of Cross-Sample Contamination: A Method by the AIRR Community.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens.Frontiers in immunology · 2022Article
- Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron.bioRxiv : the preprint server for biology · 2021Article
- Antibody screening at reduced pH enables preferential selection of potently neutralizing antibodies targeting SARS-CoV-2.AIChE journal. American Institute of Chemical Engineers · 2021Article
- Paired heavy- and light-chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses.Cell reports · 2021Article
- Sequence-Signature Optimization Enables Improved Identification of Human HV6-1-Derived Class Antibodies That Neutralize Diverse Influenza A Viruses.Frontiers in immunology · 2021Article
- Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients.Frontiers in immunology · 2021Article
- Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.Cell reports · 2020Article
- Article
- Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.bioRxiv : the preprint server for biology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Emulsion-based techniques have dramatically advanced our understanding of single-cell biology and complex single-cell features over the past two decades. Most approaches for precise single cell isolation rely on microfluidics, which has proven highly effective but requires substantial investment in equipment and expertise that can be difficult to access for researchers that specialize in other areas of bioengineering and molecular biotechnology. Inspired by the robust droplet generation technologies in modern flow cytometry instrumentation, here we established a new platform for high-throughput isolation of single cells within droplets of tunable sizes by combining flow focusing with ultrasonic vibration for rapid and effective droplet formation. Application of ultrasonic pressure waves to the flowing jet provided enhanced control of emulsion droplet size, permitting capture of 25,000 to 50,000 single cells per minute. As an example application, we applied this new droplet generation platform to sequence the antibody variable region heavy and light chain pairings (VH:VL) from large repertoires of single B cells. We demonstrated the recovery of > 40,000 paired CDRH3:CDRL3 antibody clusters from a single individual, validating that these droplet systems can enable the genetic analysis of very large single-cell populations. These accessible new technologies will allow rapid, large-scale, and precise single-cell analyses for a broad range of bioengineering and molecular biotechnology applications.
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