ArticleFood and environmental virology2019
Glass Wool Concentration Optimization for the Detection of Enveloped and Non-enveloped Waterborne Viruses.
Article in Food and environmental virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Occurrence of Hepatitis A Virus in Water Matrices: A Systematic Review and Meta-Analysis.International journal of environmental research and public health · 2023Pooled it
- Molecular exploration of mosquitoes and their vector-borne pathogens in urban water environments in Benin.Scientific reports · 2026Article
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- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Near-source passive sampling for monitoring viral outbreaks within a university residential setting.Epidemiology and infection · 2024Article
- Dynamic and Seasonal Distribution of Enteric Viruses in Surface and Well Water in Riyadh (Saudi Arabia).Pathogens (Basel, Switzerland) · 2023Article
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- Physiological characteristics, geochemical properties and hydrological variables influencing pathogen migration in subsurface system: What we know or not?Geoscience frontiers · 2022Article
- Assessment of two types of passive sampler for the efficient recovery of SARS-CoV-2 and other viruses from wastewater.The Science of the total environment · 2022Article
- A Methodological Approach to Water Concentration to Investigate the Presence of SARS-CoV-2 RNA in Surface Freshwaters.Pathogens (Basel, Switzerland) · 2022Article
- Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR.The Science of the total environment · 2022Article
- Intestinal viral infections of nSARS-CoV2 in the Indian community: Risk of virus spread in India.Journal of medical virology · 2022Review
- Recovery of SARS-CoV-2 from large volumes of raw wastewater is enhanced with the inuvai R180 system.Journal of environmental management · 2022Article
- A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal.The Science of the total environment · 2022Article
- COVID-19 surveillance in wastewater: An epidemiological tool for the monitoring of SARS-CoV-2.Frontiers in cellular and infection microbiology · 2022Review
- Diagnosis of COVID-19, vitality of emerging technologies and preventive measures.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Methods Evaluation for Rapid Concentration and Quantification of SARS-CoV-2 in Raw Wastewater Using Droplet Digital and Quantitative RT-PCR.Food and environmental virology · 2021Article
- Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications.Journal of hazardous materials · 2021Review
- Coronavirus: occurrence, surveillance, and persistence in wastewater.Environmental monitoring and assessment · 2021Review
- Impacts of COVID-19 pandemic on the wastewater pathway into surface water: A review.The Science of the total environment · 2021Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
An extremely affordable virus concentration method based on adsorption-elution to glass wool and subsequent reconcentration through polyethylene glycol 6000 (PEG) precipitation was optimized to recover not only non-enveloped viruses but also enveloped viruses. Hepatitis A virus (HAV) and transmissible gastroenteritis virus (TGEV) were employed as surrogates for naked and enveloped viruses, respectively, to set up the methodology. Initial experimentation in small-volume samples showed that both types of particles readily adsorbed to the positively charged glass wool but were poorly detached from it through standard elution with 0.05 M glycine with 3% of beef extract buffer, pH 9.5, with elution efficiencies of 7.2% and 2.6%, for HAV and TGEV, respectively. To improve the recovery of enveloped viruses, several modifications in the elution were assayed: increasing the elution pH, extending glass wool and eluent contact time, adding a detergent, or performing the elution by recirculation or under agitation. Considering practicability and performance, recircularization of the eluent at pH 11.0 for 20 min was the elution procedure of choice, with efficiencies of 25.7% and 18.8% for HAV and TGEV in 50 L of water. Additionally, employing 20% PEG instead of 10% for virus reconcentration improved recoveries up to 47% and 51%, respectively. The optimized procedure was applied to detect naturally occurring HAV and coronaviruses in surface water of Wadi Hanifa, Riyadh. HAV was detected in 38% of the samples, while one sample was positive for an alphacoronavirus. This cheap virus detection system enables the comprehensive surveillance of viruses present in water samples.
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