ArticleGenes & genomics2019
Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat.
Article in Genes & genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Cytogenomic evaluation of regenerated Aralia elata using PLOP-FISH and flow cytometry.Scientific reports · 2024Article
- Fluorescence in situ Hybridization Analysis of Oligonucleotide 5S Ribosomal DNA, 45S Ribosomal DNA, and (TTTAGGG)3 Locations in Gloriosa superba L.Cytogenetic and genome research · 2024Article
- Oligonucleotide Fluorescence In Situ Hybridization: An Efficient Chromosome Painting Method in Plants.Plants (Basel, Switzerland) · 2023Review
- Article
- FISH Mapping of Telomeric and Non-Telomeric (AGGenes · 2022Article
- Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AGGenes · 2022Article
- Article
- Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review.Plants (Basel, Switzerland) · 2021Review
- Chromosomal Characterization ofInternational journal of molecular sciences · 2021Article
- Cytogenetic analysis of Bienertia sinuspersici Akhani as the first step in genome sequencing.Genes & genomics · 2020Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe family Araliaceae contains many medicinal species including ginseng of which the whole genome sequencing analyses have been going on these days.
objectiveTo characterize the chromosomal distribution of 5S and 45S rDNAs and telomeric repeat in four ginseng related species of Aralia elata (Miq.) Seem., Dendropanax morbiferus H. Lév., Eleutherococcus sessiliflorus (Rupr. Et Maxim.) Seem., Kalopanax septemlobus (Thunb. ex A.Murr.) Koidz.
methodPre-labelled oligoprobe (PLOP)-fluorescence in situ hybridization (FISH) was carried out.
resultsThe chromosome number of A. elata was 2n = 24, whereas that of the other three species of D. morbiferus, E. sessiliflorus, and K. septemlobus was 2n = 48, corresponding to diploid and tetraploid, respectively, based on the basic chromosome number x = 12 in Araliaceae. In all four species, one pair of 5S signals were detected in the proximal regions of the short arms of chromosome 3, whereas in K. septemlobus, the 5S rDNA signals localized in the subtelomeric region of short arm of chromosome 3, while all the 45S rDNA signals localized at the paracentromeric region of the short arm of chromosome 1. And the telomeric repeat signals were detected at the telomeric region of both short and long arms of most chromosomes.
conclusionThe PLOP-FISH was very effective compared with conventional FISH method. These results provide useful comparative cytogenetic information to better understand the genome structure of each species and will be useful to trace the history of ginseng genomic constitution.
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