ArticleNature protocols2019
High-resolution 3D imaging and analysis of axon regeneration in unsectioned spinal cord with or without tissue clearing.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Technological advances in axonal regeneration and structural plasticity: optogenetics, single-cell omics, and tissue clearing.Frontiers in cellular neuroscience · 2026Review
- Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- In vivo programming of adult pericytes aids axon regeneration by providing cellular bridges for SCI repair.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Differential Expression of Disulfidptosis-Related Genes in Spinal Cord Injury and Their Role in the Immune Microenvironment.Molecular neurobiology · 2025Article
- High-Speed Clearing and High-Resolution Staining for Analysis of Various Markers for Neurons and Vessels.Tissue engineering and regenerative medicine · 2024Article
- Transplantation of dorsal root ganglia overexpressing the NaChBac sodium channel improves locomotion after complete SCI.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- A Versatile Pipeline for High-fidelity Imaging and Analysis of Vascular Networks Across the Body.Bio-protocol · 2024Article
- Tissue optical clearing for neural regeneration research.Neural regeneration research · 2023Article
- Mechanisms of Axon Growth and Regeneration: Moving between Development and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Harnessing cortical plasticity via gabapentinoid administration promotes recovery after stroke.Brain : a journal of neurology · 2022Article
- An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.Neuron · 2022Article
- α-Synuclein Impacts on Intrinsic Neuronal Network Activity Through Reduced Levels of Cyclic AMP and Diminished Numbers of Active Presynaptic Terminals.Frontiers in molecular neuroscience · 2022Article
- Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury.Science (New York, N.Y.) · 2021Article
- Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord.Cell reports methods · 2021Article
- Diversity of Reactive Astrogliosis in CNS Pathology: Heterogeneity or Plasticity?Frontiers in cellular neuroscience · 2021Review
- Passive Clearing and 3D Lightsheet Imaging of the Intact and Injured Spinal Cord in Mice.Frontiers in cellular neuroscience · 2021Article
- Source of Early Regenerating Axons in Lamprey Spinal Cord Revealed by Wholemount Optical Clearing with BABB.Cells · 2020Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we present a protocol for analyses of axon regeneration and density in unsectioned adult mouse spinal cord. This includes methods for injury and tracing of dorsal column sensory and corticospinal axons; clearing and staining of unsectioned spinal cord; visualization of axon degeneration and regeneration in cleared and uncleared specimens using two-photon microscopy; and either manual or semi-automatic analysis of axon density and regeneration in 3D space using Imaris and ImageJ software. This protocol can be used to elucidate the molecular and cellular mechanisms underlying nervous system degeneration and regeneration and to establish the therapeutic efficacy of candidate neuroregenerative treatments. Because tissue sectioning is not required, this protocol enables unambiguous evaluation of regeneration and greatly accelerates the speed at which analyses can be conducted. Surgical procedures take <30 min per mouse, with a wait period of 2 weeks between axonal injury and tracing and 2-8 weeks between tracing and tissue processing. Clearing and immunolabeling take ~1-2 weeks, depending on the size of the sample. Imaging and analysis can be performed in 1 d. All these procedures can be accomplished by a competent graduate student or experienced technician.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.