ArticleNature protocols2019
Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 114 citations in OpenAlex.
- Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects.Biophysics reports · 2026Article
- Decoding the Hot-Mitochondrion Paradox.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Dual Minimization of Spectrum Overlap for High-Sensitivity, High-Temperature Sensing.Sensors (Basel, Switzerland) · 2025Article
- Magnetic nanomaterials for hyperthermia-based therapy and controlled drug delivery.Bioactive materials · 2025Review
- Effect of NaCl on the luminescent behavior of CsI thin films.RSC advances · 2025Article
- Multiplexed quantum sensing reveals coordinated thermomagnetic regulation of mitochondria.bioRxiv : the preprint server for biology · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Exploring the Frontiers of Cell Temperature Measurement and Thermogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers.Beilstein journal of nanotechnology · 2025Article
- Mitochondrial Thermogenesis Can Trigger Heat Shock Response in the Nucleus.ACS central science · 2024Article
- Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations.ACS photonics · 2023Article
- Nanoparticles for Interrogation of Cell Signaling.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Local Temperature Increments and Induced Cell Death in Intracellular Magnetic Hyperthermia.ACS nano · 2023Article
- Single-pulse real-time billion-frames-per-second planar imaging of ultrafast nanoparticle-laser dynamics and temperature in flames.Light, science & applications · 2023Article
- Measurement of Intracellular Temperature in Brown Adipocytes Using a Cationic Fluorescent Polymeric Thermometer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing.Biosensors · 2022Article
- Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis.Journal of the American Chemical Society · 2022Article
- Do compromised mitochondria aggravate severity and fatality by SARS-CoV-2?Current medical research and opinion · 2022Article
- Advantages and Limitations of Fluorescence Lifetime Measurements Using Single-Photon Avalanche Diode (SPAD) Array Detector: A Comprehensive Theoretical and Experimental Study.Sensors (Basel, Switzerland) · 2022Article
- High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts.ACS photonics · 2022Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temperature is one of the most important of the physiological parameters that determine the biological status of living organisms. However, intracellular temperature was not imaged at the single-cell level until recently because of the lack of a molecular thermometer that can be applied to living cells. We have recently developed a method for imaging intracellular temperature using a cationic linear fluorescent polymeric thermometer (FPT) and fluorescence lifetime imaging microscopy (FLIM). The cationic linear FPT exhibits cell permeability in various mammalian cell lines and yeast cells, entering live cells within 10 min of incubation. Intracellular thermometry using the cationic linear FPT and FLIM can be used to image temperature with high temperature resolution (0.3-1.29 °C within a temperature range of 25-35 °C). The diffuse intracellular localization of the cationic linear FPT allows a high spatial resolution (i.e., the light microscope's diffraction limit, 200 nm), enabling the detection of temperature distributions at the subcellular level. This protocol, including the construction of a calibration curve and intracellular temperature imaging, requires ~14 h. Experience in handling cultured mammalian cells and use of a confocal laser-scanning microscope (CLSM) is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.