ArticleScientific reports2019
Novel genetic tools that enable highly pure protein production in Trichoderma reesei.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Efficient Extracellular Production of Insulin Glargine in Trichoderma reesei by an Integrative Secretion-Based Strategy.Microbial biotechnology · 2026Article
- Engineering heterologous core promoters to optimize the SES expression system forSynthetic and systems biotechnology · 2026Article
- Oxyfunctionalisation of anisole and its selected reaction products by unspecific peroxygenases.Biochemistry and biophysics reports · 2025Article
- Improving recombinant protein secretion inMetabolic engineering communications · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Label-free analytical characterization of brazzein produced with the filamentous fungusFrontiers in bioengineering and biotechnology · 2025Article
- Disentangling the Web: An Interdisciplinary Review on the Potential and Feasibility of Spider Silk Bioproduction.ACS biomaterials science & engineering · 2024Review
- Transcriptomic and metabolic changes in Trichoderma reesei caused by mutation in xylanase regulator 1 (xyr1).Biotechnology for biofuels and bioproducts · 2024Article
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production.Biotechnology for biofuels and bioproducts · 2024Article
- Heterologous protein production in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.Microbial cell factories · 2023Article
- Enhancing the production of a heterologous Trametes laccase (LacA) by replacement of the major cellulase CBH1 in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- A multi-plex protein expression system for production of complex enzyme formulations in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- Engineering cellulases for conversion of lignocellulosic biomass.Protein engineering, design & selection : PEDS · 2023Review
- Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichoderma reesei.Scientific reports · 2022Article
- Non-Mammalian Eukaryotic Expression Systems Yeast and Fungi in the Production of Biologics.Journal of fungi (Basel, Switzerland) · 2022Review
- Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.Bioresources and bioprocessing · 2022Article
- Use of CRISPR-Cas tools to engineer Trichoderma species.Microbial biotechnology · 2022Review
- Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review.Journal of fungi (Basel, Switzerland) · 2022Review
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Authors and funding
6 authors.
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Abstract
Trichoderma reesei is an established protein production host with high natural capacity to secrete enzymes. The lack of efficient genome engineering approaches and absence of robust constitutive gene expression systems limits exploitation of this organism in some protein production applications. Here we report engineering of T. reesei for high-level production of highly enriched lipase B of Candida antarctica (calB) using glucose as a carbon source. Multiplexed CRISPR/Cas9 in combination with the use of our recently established synthetic expression system (SES) enabled accelerated construction of strains, which produced high amounts of highly pure calB. Using SES, calB production levels in cellulase-inducing medium were comparable to the levels obtained by using the commonly employed inducible cbh1 promoter, where a wide spectrum of native enzymes were co-produced. Due to highly constitutive expression provided by the SES, it was possible to carry out the production in cellulase-repressing glucose medium leading to around 4 grams per liter of fully functional calB and simultaneous elimination of unwanted background enzymes.
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