ArticleNature communications2019
Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 88 citations in OpenAlex.
- Engineering strategies for the biosynthesis of rare sugars in microbial cell factories.World journal of microbiology & biotechnology · 2026Review
- Model-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.Microbial cell factories · 2026Article
- Engineering ofCurrent research in food science · 2026Article
- Reversal of the Leloir pathway to promote galactose and tagatose synthesis from glucose.Cell reports. Physical science · 2025Article
- Identification, characterization, and application of an NADPH-dependent carbonyl reductase inSynthetic and systems biotechnology · 2025Article
- Activating the d-Tagatose Production Capacity ofJournal of agricultural and food chemistry · 2025Article
- Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis.AMB Express · 2025Article
- Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway.Applied microbiology and biotechnology · 2025Article
- Transforming monosaccharides: Recent advances in rare sugar production and future exploration.BBA advances · 2025Article
- A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeastApplied and environmental microbiology · 2024Article
- Stereoselective Solid-State Synthesis of Biologically Active Cyclobutane and Dicyclobutane Isomers via Conformation Blocking and Transference.Molecules (Basel, Switzerland) · 2024Article
- A hybrid photocatalytic system enables direct glucose utilization for methanogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Awakening the natural capability of psicose production in Escherichia coli.NPJ science of food · 2023Article
- Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone.Biotechnology and bioengineering · 2022Article
- Investigating the role of the transcriptional regulator Ure2 on the metabolism of Saccharomyces cerevisiae: a multi-omics approach.Applied microbiology and biotechnology · 2021Article
- Supramolecular-induced regiocontrol over the photochemical [4 + 4] cyclodimerization of NHC- or azole-substituted anthracenes.Chemical science · 2020Article
- Genome-scale metabolic reconstruction of the non-model yeastMetabolic engineering communications · 2020Article
- Xylose utilization in Saccharomyces cerevisiae during conversion of hydrothermally pretreated lignocellulosic biomass to ethanol.Applied microbiology and biotechnology · 2020Review
- Biological upgrading of 3,6-anhydro-L-galactose from agarose to a new platform chemical.Green chemistry : an international journal and green chemistry resource : GC · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isomerases perform biotransformations without cofactors but often cause an undesirable mixture of substrate and product due to unfavorable thermodynamic equilibria. We demonstrate the feasibility of using an engineered yeast strain harboring oxidoreductase reactions to overcome the thermodynamic limit of an isomerization reaction. Specifically, a yeast strain capable of consuming lactose intracellularly is engineered to produce tagatose from lactose through three layers of manipulations. First, GAL1 coding for galactose kinase is deleted to eliminate galactose utilization. Second, heterologous xylose reductase (XR) and galactitol dehydrogenase (GDH) are introduced into the ∆gal1 strain. Third, the expression levels of XR and GDH are adjusted to maximize tagatose production. The resulting engineered yeast produces 37.69 g/L of tagatose from lactose with a tagatose and galactose ratio of 9:1 in the reaction broth. These results suggest that in vivo oxidoreaductase reactions can be employed to replace isomerases in vitro for biotransformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.