ArticleNature communications2019
Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 54 citations in OpenAlex.
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- Roadmap on computational methods in optical imaging and holography [invited].Applied physics. B, Lasers and optics · 2024Review
- Computational optics for high-throughput imaging of neural activity.Neurophotonics · 2022Review
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Recurrent neural network-based volumetric fluorescence microscopy.Light, science & applications · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination patterns are combined with the saturation of an optical transition to enable sub-diffraction imaging. While the technique proved useful for two-dimensional imaging, extending it to three-dimensions is challenging due to the fading of organic fluorophores under intense cycling conditions. Here, we present a compressed sensing approach that allows 3D sub-diffraction nSIM of cultured cells by saturating fluorescence excitation. Exploiting the natural orthogonality of speckles at different axial planes, 3D probing of the sample is achieved by a single two-dimensional scan. Fluorescence contrast under saturated excitation is ensured by the inherent high density of intensity minima associated with optical vortices in polarized speckle patterns. Compressed speckle microscopy is thus a simple approach that enables 3D super-resolved nSIM imaging with potentially considerably reduced acquisition time and photobleaching.
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Registered trials
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