ArticleBMC musculoskeletal disorders2019
Molecular changes to tendons after collagenase-induced acute tendon injury in a senescence-accelerated mouse model.
Article in BMC musculoskeletal disorders, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Tendon Aging: A Silent Enemy Revealed Strategies for Effective Treatment.Aging medicine (Milton (N.S.W)) · 2025Review
- Establishment of a Mouse Degenerative Model of Patellar Tendinopathy with Upregulation of Inflammation.International journal of molecular sciences · 2024Article
- A Novel Tendon Injury Model, Induced by Collagenase Administration Combined with a Thermo-Responsive Hydrogel in Rats, Reproduces the Pathogenesis of Human Degenerative Tendinopathy.International journal of molecular sciences · 2024Article
- Interleukin-6 upregulates extracellular matrix gene expression and transforming growth factor β1 activity of tendon progenitor cells.BMC musculoskeletal disorders · 2023Article
- Mammal comparative tendon biology: advances in regulatory mechanisms through a computational modeling.Frontiers in veterinary science · 2023Article
- An overview of the material science and knowledge of nanomedicine, bioscaffolds, and tissue engineering for tendon restoration.Frontiers in bioengineering and biotechnology · 2023Review
- Inhibitory effect of Astragalus Membranaceus on osteoporosis in SAMP6 mice by regulating vitaminD/FGF23/Klotho signaling pathway.Bioengineered · 2021Article
- Tendon Extracellular Matrix Assembly, Maintenance and Dysregulation Throughout Life.Advances in experimental medicine and biology · 2021Review
- Reparative and Maladaptive Inflammation in Tendon Healing.Frontiers in bioengineering and biotechnology · 2021Review
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Umbilical cord-derived Wharton's jelly for regenerative medicine applications.Journal of orthopaedic surgery and research · 2020Article
- Models of tendon development and injury.BMC biomedical engineering · 2019Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAging impairs tendon healing and is a potential risk factor for chronic tendinitis. During normal aging, tendons undergo structural and biomechanical degenerative changes, accompanied by a reduction in the number of tenocytes and changes to their properties. However, molecular changes in aged tendons under inflammatory conditions are not well understood. The present study analyzed the molecular changes in collagenase induced acute tendon injury using a senescence-accelerated mouse (SAM) model.
methodsSAMP6 mice were used as an aging animal model and SAMR1 mice were used as a control to represent a senescence-resistant inbred strain. All the mice used in the study were 40 weeks old. Collagenase I from Clostridium histolyticum (20 μL) was injected percutaneously to the tendon-bone junction of the Achilles tendon. Two weeks after treatment, the Achilles tendons were harvested and stained using Picrosirius Red to determine collagen expression. Real-time PCR was performed to analyze gene expression of IL-6, tenomodulin, type I and type II collagen, MMP-9, TIMP-1, and TIMP-2.
resultsCollagenase injection resulted in significantly higher gene expression of IL-6 but significantly lower tenomodulin expression compared with the control in SAMP6 and SAMR1 mice. In SAMP6 mice, gene expression of type III collagen and MMP-9 was significantly higher in the collagenase-injected group compared with the control group. SAMP6 mice also showed lower expression of type I collagen, TIMP-1, and TIMP-2 in the collagenase-injected group compared with the control group. Picrosirius Red staining showed the highest expression of type III collagen in the collagenase-injected SAMP6 group compared with the other groups.
conclusionsThe collagenase-injected SAMP6 group showed higher expression of IL-6, MMP-9, and type III collagen and lower expression of type I collagen, TIMP-1, and TIMP-2, which are known to suppress metalloproteinases. The results indicate that aging may lead to dysfunction of the tendon healing process after acute tendon injury.
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