ArticleMetabolites2019
Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs.
Article in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Differentiated short- and long-term impacts of a starter stimbiotic supplementation on gut health in broilers fed wheat and rye-based diets at homeostasis and underAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.Communications biology · 2024Article
- Gut-Brain-axis: effect of basil oil on the gut microbiota and its contribution to the anticonvulsant properties.BMC complementary medicine and therapies · 2023Article
- Gut microbiota short-chain fatty acids and their impact on the host thyroid function and diseases.Frontiers in endocrinology · 2023Review
- Lactobacillus rhamnosus GG modifies the metabolome of pathobionts in gnotobiotic mice.BMC microbiology · 2021Article
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Authors and funding
7 authors.
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Abstract
Nuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparatively easy in NMR metabolomics, because mainly buffer and chemical shift reference substance are added. For solid samples like feces suitable extraction protocols need to be defined as initial step, where the exact protocol depends on sample type and features. Focusing on short chain fatty acids (SCFAs) in mice feces, we describe here a set of extraction protocols developed with the aim to suppress changes in metabolite composition within 24 h after extraction. Feces are obtained from mice fed on either standard rodent diet or high fat diet. The protocols presented in this manuscript are straightforward for application, and successfully minimize residual bacterial and enzymatic activities. Additionally, they are able to minimize the lipid background originating from the high fat diet.
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