ArticleMolecules (Basel, Switzerland)2019
The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH.
Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 16 citations in OpenAlex.
- Frequent variations and phylogenetic relationships within the genusFrontiers in plant science · 2024Article
- Chromosomal analysis of progenies betweenFrontiers in plant science · 2024Article
- Development and Molecular Cytogenetic Characterization of a Novel Wheat-Rye T6RS.6AL Translocation Line fromInternational journal of molecular sciences · 2022Article
- Developing Oligo Probes for Chromosomes Identification in Hemp (Plants (Basel, Switzerland) · 2022Article
- Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization.Plants (Basel, Switzerland) · 2022Article
- Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line fromInternational journal of molecular sciences · 2022Article
- Identification and validation of quantitative trait loci for the functional stay green trait in common wheat (Triticum aestivum L.) via high-density SNP-based genotyping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Molecular Cytogenetic Characterization of Novel 1RS.1BL Translocation and Complex Chromosome Translocation Lines with Stripe Rust Resistance.International journal of molecular sciences · 2022Article
- Molecular cytogenetic identification of new wheat-rye 6R, 6RS, and 6RL addition lines with resistance to stripe rust and powdery mildew.Frontiers in plant science · 2022Article
- Preferential Subgenome Elimination and Chromosomal Structural Changes Occurring in Newly Formed Tetraploid Wheat-Frontiers in genetics · 2020Article
- New ND-FISH-Positive Oligo Probes for IdentifyingInternational journal of molecular sciences · 2019Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21 wheat cultivars and lines. These oligonucleotide probes distinguished 42 wheat chromosomes and also detected rye chromatin in the wheat genome. Moreover, the signal patterns of the oligonucleotide probes Oligo-pTa535-1 and Oligo-pSc119.2-1 showed high polymorphism in the wheat chromosomes. A total of 17.6% of the A group chromosomes, 25.9% of the B group chromosomes and 8.9% of the D group chromosomes showed obvious mutations when they were compared to the standard ND-FISH signal patterns, and most of them were Oligo-pSc119.2-1 mutants. The results suggested that these polymorphisms could be induced by the crossing of wheat cultivars. The results provided more information for the further application of oligonucleotide probes and ND-FISH.
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