Evidence map›Paper›PMID 30901855›Full record

ArticleAnimals : an open access journal from MDPI2019

Optimization of In Vitro Culture Conditions of Sturgeon Germ Cells for Purpose of Surrogate Production.

Xuan Xie, Ping Li, Martin Pšenička, Huan Ye, Christoph Steinbach, Chuangju Li, Qiwei Wei

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 20 citations in OpenAlex.

  1. Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Optimization ofFrontiers in veterinary science · 2022
    Article
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Xuan XieKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. xxie@jcu.cz.
Ping LiResearch Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 38925 Vodňany, Czech Republic. liping2018@email.sdu.edu.cn.ORCID 0000-0002-6931-8610
Martin PšeničkaResearch Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 38925 Vodňany, Czech Republic. psenicka@frov.jcu.cz.
Huan YeKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. yehuan85@163.com.
Christoph SteinbachResearch Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 38925 Vodňany, Czech Republic. steinbach@frov.jcu.cz.
Chuangju LiKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. lcj@yfi.ac.cn.
Qiwei WeiKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. weiqw@yfi.ac.cn.
University of South Bohemia in České Budějovice · CZChinese Academy of Fishery Sciences · CN

Funding

Czech Science Foundation 16-02407YMinistry of Education, Youth and Sports of the Czech Republic CZ.1.05/2.1.00/01.0024Ministry of Education, Youth and Sports of the Czech Republic LO1205National Natural Science Foundation of China 31402301Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China 201203086Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences 2015B02YQ01
6 · The paper itself

Abstract

To expand germ cell populations and provide a consistent supply for transplantation, we established basal culture conditions for sturgeon germ cells and subsequently increased their mitotic activity by eliminating gonad somatic cells, supplementing with growth factor, and replacing fetal bovine serum (FBS). The initial basal culture conditions were Leibovitz's L-15 medium (pH 8.0) supplemented with 5% FBS (

Indexed as

feeder cellsgerm cell cultureglial-cell-derived neurotrophic factorsturgeontransplantation

Identifiers

PMID30901855
PMCPMC6466142
OpenAlexW2923481750

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.