ArticleMetabolomics : Official journal of the Metabolomic Society2019
Evaluation of metabolome sample preparation and extraction methodologies for oleaginous filamentous fungi Mortierella alpina.
Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 32 citations in OpenAlex.
- Application of Metabolomics in the Exploration of Bioactive Compounds and Drug Development from Medicinal Fungi.Metabolites · 2026Review
- Roles of the Fungal-Specific Lysine Biosynthetic Pathway in the Nematode-Trapping FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Sample Preparation in Microbial Metabolomics: Advances and Challenges.Advances in experimental medicine and biology · 2023Article
- Function discovery of a non-ribosomal peptide synthetase-like encoding gene in the nematode-trapping fungusFrontiers in microbiology · 2023Article
- Application of Metabolomics in Fungal Research.Molecules (Basel, Switzerland) · 2022Review
- Evaluation and optimization of analytical procedure and sample preparation for polarSynthetic and systems biotechnology · 2022Article
- Streptococcus mutans and Candida albicans Biofilm Inhibitors Produced by Lactiplantibacillus plantarum CCFM8724.Current microbiology · 2022Article
- Autophagy Improves ARA-Rich TAG Accumulation inMicrobiology spectrum · 2022Article
- Multi-Omics Reveals the Inhibition ofMicroorganisms · 2021Article
- Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.Biotechnology letters · 2021Article
- Carbohydrate analysis of Mortierella alpina by colorimetry and HPLC-ELSD to reveal accumulation differences of sugar and lipid.Biotechnology letters · 2021Article
- Time-resolved multi-omics analysis reveals the role of nutrient stress-induced resource reallocation for TAG accumulation in oleaginous fungusBiotechnology for biofuels · 2020Article
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenases in NADPH Supply in the Oleaginous Filamentous FungusFrontiers in microbiology · 2020Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMetabolomics has been successfully applied to guide the rational engineering of industrial strains and improve the performance of bioprocesses. Mortierella alpina has traditionally been one of the most popular industrial strains for the production of polyunsaturated fatty acids. However, a systematic comparison and optimisation of the metabolomic analysis methods of M. alpina has not yet been reported.
objectiveWe sought to identify potential weaknesses that are important for accurate metabolomic analysis. We also aimed to determine an efficient sample preparation protocol for metabolomics studies in the oleaginous filamentous fungus M. alpina.
methodsIn this study, using GC-MS, we evaluated three sample preparation protocols and five solvent mixtures by assessment of the metabolite profile differences, the sum of peak intensities and the reproducibility of metabolite quantification.
resultsThe freeze-dried biomass had better reproducibility and recovery than fresh biomass for metabolite extraction and data normalisation that is part of a metabolomics analysis of filamentous fungi M. alpina. Methanol:water (1:1) was superior for the profiling of metabolites in oleaginous fungi M. alpina. The unbiased metabolite profiling difference between the growth phase and lipids synthesis phase revealed that the degradation of amino acids were critical nodes for the efficient synthesis of lipids in M. alpina.
conclusionThe use of freeze-dried biomass for metabolite extraction and data normalisation was more efficient at measuring the active state of the intracellular metabolites in M. alpina. We recommend extracting the intracellular metabolites with methanol:water (1:1). An important role of amino acid oxidation in the nitrogen limitation-mediated lipid accumulation was found.
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