ArticlePlant methods2019
Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Stress-induced MAPK (SIMK)-dependent organization of microtubules in alfalfa.Molecular horticulture · 2026Article
- FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques.Plant methods · 2026Article
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- New insights into the functions and regulations of MAP215/MOR1 and katanin, two conserved microtubule-associated proteins in Arabidopsis.Plant signaling & behavior · 2023Article
- A MOF/DNA luminescent sensing platform for detection of potential COVID-19 biomarkers and drugs.Chemical science · 2023Article
- Cortical microtubules contribute to division plane positioning during telophase in maize.The Plant cell · 2023Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- Binding of Tau-derived peptide-fused GFP to plant microtubules in Arabidopsis thaliana.PloS one · 2023Article
- Imaging plant cells and organs with light-sheet and super-resolution microscopy.Plant physiology · 2022Article
- Membrane imaging in the plant endomembrane system.Plant physiology · 2021Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Spatiotemporal Pattern of Ectopic Cell Divisions Contribute to Mis-Shaped Phenotype of Primary and Lateral Roots ofFrontiers in plant science · 2020Article
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Authors and funding
8 authors.
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Abstract
backgroundIn the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a considerable z-range and circumvent its low temporal resolution capacity during the study of living samples. Further, we validate the protocol for the imaging of fixed transgenic material expressing fluorescent protein-based markers of different subcellular structures.
resultsFocus is given on 3D imaging of bulky subcellular structures, such as mitotic and cytokinetic microtubule arrays as well as on the performance of SIM using multichannel imaging and the quantitative correlations that can be deduced. As a proof of concept, we provide a superresolution output on the organization of cortical microtubules in wild-type and mutant Arabidopsis cells, including aberrant preprophase microtubule bands and phragmoplasts in a cytoskeletal mutant devoid of the p60 subunit of the microtubule severing protein KATANIN and refined details of cytoskeletal aberrations in the mitogen activated protein kinase (MAPK) mutant
conclusions3D SIM is a powerful, versatile and adaptable microscopy method for elucidating spatial relationships between subcellular compartments. Improved methods of sample preparation aiming to the compensation of refractive index mismatches, allow the use of 3D SIM in the documentation of complex plant cell structures, such as microtubule arrays and the elucidation of their interactions with microtubule associated proteins.
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