ArticleEmerging microbes & infections2019
Cultivation of the causative agent of human neoehrlichiosis from clinical isolates identifies vascular endothelium as a target of infection.
Article in Emerging microbes & infections, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Neoehrlichiosis Causing Hemolytic Anemia in Patients Treated with Rituximab, Norway, 2024-2025.Emerging infectious diseases · 2026Article
- Latent Neoehrlichia mikurensis Infections May Be Reactivated in Patients With B-Cell Lymphomas Treated With Rituximab.Immunology · 2026Article
- Detection of human antibody responses to tick-borne Neoehrlichia mikurensis.Medical microbiology and immunology · 2026Article
- Neoehrlichia mikurensis in Ticks and Tick-Bitten Persons, Sweden and Finland, 2008-2009.Emerging infectious diseases · 2025Article
- Tickborne Neoehrlichia mikurensis in the Blood of Blood Donors, Norway, 2023.Emerging infectious diseases · 2025Article
- Neoehrlichia mikurensis-associated secondary capillary leak syndrome and septic shock: a case report.Journal of medical case reports · 2025Article
- Isolation, Molecular Characterization and In Vitro Propagation of anPathogens (Basel, Switzerland) · 2025Article
- Protective Effects of Luteolin onAntibiotics (Basel, Switzerland) · 2025Article
- Geographic Variation in the Prevalence of Candidatus Neoehrlichia procyonis in Raccoons (Procyon lotor) in the United States and Canada.MicrobiologyOpen · 2025Article
- Ten years of detecting Neoehrlichia mikurensis infections in Sweden: demographic, clinical and inflammatory parameters.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Ticks and Tick-Borne Pathogens Circulating in Peri-Domestic Areas in Mainland Portugal.Microorganisms · 2024Article
- Article
- Detection and phylogenetic classification ofFrontiers in microbiology · 2024Article
- Candidatus Neoehrlichia mikurensis Infection in Patient with Antecedent Hematologic Neoplasm, SpainEmerging infectious diseases · 2023Article
- Fever of Unknown Origin, a Vascular Event, and Immunosuppression in Tick-Endemic Areas: Think About Neoehrlichiosis.Cureus · 2023Article
- Bacteria and protozoa with pathogenic potential in Ixodes ricinus ticks in Viennese recreational areas.Wiener klinische Wochenschrift · 2023Article
- The Tick-Borne Pathogens: An Overview of China's Situation.Acta parasitologica · 2023Review
- Article
- Co-Infection of Potential Tick-Borne Pathogens of the Order Rickettsiales andMicroorganisms · 2023Article
- Hazard potential of Swiss Ixodes ricinus ticks: Virome composition and presence of selected bacterial and protozoan pathogens.PloS one · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candidatus (Ca.) Neoehrlichia mikurensis is the cause of neoehrlichiosis, an emerging tick-borne infectious disease characterized by fever and vascular events. The bacterium belongs to the Anaplasmataceae, a family of obligate intracellular pathogens, but has not previously been cultivated, and it is uncertain which cell types it infects. The goals of this study were to cultivate Ca. N. mikurensis in cell lines and to identify possible target cells for human infection. Blood components derived from infected patients were inoculated into cell lines of both tick and human origin. Bacterial growth in the cell cultures was monitored by real-time PCR and imaging flow cytometry. Ca. N. mikurensis was successfully propagated from the blood of immunocompromised neoehrlichiosis patients in two Ixodes spp. tick cell lines following incubation periods of 7-20 weeks. Human primary endothelial cells derived from skin microvasculature as well as pulmonary artery were also susceptible to infection with tick cell-derived bacteria. Finally, Ca. N. mikurensis was visualized within circulating endothelial cells of two neoehrlichiosis patients. To conclude, we report the first successful isolation and propagation of Ca. N. mikurensis from clinical isolates and identify human vascular endothelial cells as a target of infection.
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