ArticlePloS one2019
Effect of preservation on fish morphology over time: Implications for morphological studies.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 43 citations in OpenAlex.
- Non-lethal imaging and modeling approaches for estimating dry mass in aquatic larvae.PloS one · 2026Article
- Temporal Effects of Preservation on the Shape and Size of Yellow Perch: Implications for Morphological Analyses.Ecology and evolution · 2025Article
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- Optical coherence tomography as an alternative tool for evaluating the effects of glyphosate on hybrid catfish (Toxicology reports · 2022Article
- Eye size and investment in frogs and toads correlate with adult habitat, activity pattern and breeding ecology.Proceedings. Biological sciences · 2020Article
- In the foothill zone-Ecology and evolution · 2020Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has long been recognized that the process of preserving biological specimens results in alterations of body shape, though detailed studies examining the degree to which morphological changes occur throughout the preservation process are lacking. We utilize geometric morphometric analyses, an increasingly common tool for examining shape variation in a wide variety of biological disciplines, to examine the effects of formalin and ethanol preservation on the body shape of 10 freshwater fish species over time: from fresh specimens to eight weeks after preservation. We found significant changes in body shape among fresh and formalin fixed specimens. Furthermore, changes in body shape continue to occur after subsequent ethanol preservation. Two fish species collected at multiple localities show significant morphological differences for a limited number of morphometric characters. However, the significance, or lack thereof, often changed inconsistently from one stage of preservation to another. We conclude that morphometric analyses would ideally be performed on fresh specimens. However, recognizing that this is not always feasible, it is important to be aware of the morphometric changes that can occur during preservation.
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Registered trials
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