ArticleMolecular medicine reports2019
TGF‑β1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway.
Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 66 citations in OpenAlex.
- Osteogenic Differentiation Induced by Dental Pulp Stem Cells Secretome: A Dose-Dependent Comparative Study.Clinical and experimental dental research · 2026Article
- Development-based In Vivo Bioreactor Strategy for Challenging Senescent Bone Reconstruction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Reduced osteogenic factors and early osteoblast senescence in SOD1(G93A) ALS mouse model.JCI insight · 2026Article
- Review
- Induced membrane-mediated DBM in combination with PRP: an autologous bone graft substitute therapy with remarkable osteoformation potency for challenging bone defects.International journal of surgery (London, England) · 2026Article
- Enhanced Osteogenic and Neurogenic Differentiation of Dental Pulp Stem Cells by Micro-Current Stimulation and Chitosan-Poly(Ethylene Glycol) Blended Calcium Phosphate Cement.ACS applied bio materials · 2026Article
- Protective effects and mechanism of chrysophanol against age-related osteoporosis in rats.Iranian journal of basic medical sciences · 2026Article
- Que/n-HA/PGCL microspheres orchestrate bone repair via context-dependent osteoimmune mechanisms: a transcriptomic dissection of direct and immune-mediated signaling.Frontiers in immunology · 2026Article
- Bingqing Gao Facilitates the Healing Process of Full-Thickness Skin Defects in Rat Wounds by Activating the PI3K/AKT Pathway.Combinatorial chemistry & high throughput screening · 2026Article
- Revolutionizing osteoporosis management: the emerging role of immunotherapeutic and next-generation therapies.Inflammopharmacology · 2025Review
- Concentrations of adrenocorticotropic hormone too low to effect cortisol enhance osteogenesis in vitro and in vivo.American journal of physiology. Endocrinology and metabolism · 2025Article
- Association between lncRNA H19 and osteoporosis in postmenopausal patients with type 2 diabetes mellitus.Journal of orthopaedic surgery and research · 2025Article
- Nanomaterial-based scaffolds for bone regeneration with piezoelectric properties.Nanomedicine (London, England) · 2025Review
- Nanomaterial application for protein delivery in bone regeneration therapy.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Review
- PI3K/AKT signaling mediate collagen type 1-induced osteogenic differentiation of dental pulp stem cells via focal adhesion mechanism.Journal of applied oral science : revista FOB · 2025Article
- Research progress on osteoclast regulation by biodegradable magnesium and its mechanism.Regenerative biomaterials · 2025Review
- Undemineralized dentin matrix particles accelerate blood vessel formation in a critical-sized skull defect through activating the TGF-β/PI3K signaling pathway.American journal of translational research · 2025Article
- Investigating the potential effect of Holothuria scabra extract on osteogenic differentiation in preosteoblast MC3T3-E1 cells.Scientific reports · 2024Article
- Platelets in Thrombosis and Atherosclerosis: A Double-Edged Sword.The American journal of pathology · 2024Review
- The balance between helper T 17 and regulatory T cells in osteoimmunology and relevant research progress on bone tissue engineering.Immunity, inflammation and disease · 2024Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transforming growth factor β1 (TGF‑β1) has been suggested to be a candidate cytokine in the field of bone tissue engineering. Cytokines serve important roles in tissue engineering, particularly in the repair of bone damage; however, the underlying molecular mechanisms remain unclear. In the present study, the effects of TGF‑β1 on the osteogenesis and motility of hFOB1.19 human osteoblasts were demonstrated via the phenotype and gene expression of cells. Additionally, the role of the phosphatidylinositol 3‑kinase/protein kinase B/mammalian target of rapamycin/S6 kinase 1 (PI3K/AKT/mTOR/S6K1) signalling pathway in the effects of TGF‑β1 on osteoblasts was investigated. It was demonstrated using Cell Counting Kit‑8 and flow cytometry assays that the proliferation of human osteoblasts was promoted by 1 ng/ml TGF‑β1. In addition, alkaline phosphatase activity, Alizarin red staining, scratch‑wound and Transwell assays were conducted. It was revealed that osteogenesis and the migration of cells were regulated by TGF‑β1 via the upregulation of osteogenic and migration‑associated genes. Alterations in the expression of osteogenesis‑ and migration‑associated genes were evaluated following pre‑treatment with a PI3K/AKT inhibitor (LY294002) and an mTOR/S6K1 inhibitor (rapamycin), with or without TGF‑β1. The results indicated that TGF‑β1 affected the osteogenesis and mineralisation of osteoblasts via the PI3K/AKT signalling pathway. Furthermore, TGF‑β1 exhibited effects on mTOR/S6K1 downstream of PI3K/AKT. The present study demonstrated that TGF‑β1 promoted the proliferation, differentiation and migration of human hFOB1.19 osteoblasts, and revealed that TGF‑β1 affected the biological activity of osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Our findings may provide novel insight to aid the development of bone tissue engineering methods for the treatment of bone injury.
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