ArticleCell proliferation2019
Characterization of porcine extraembryonic endoderm cells.
Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- BMP4 and FGF2 promote heterogeneous priming including extraembryonic lineages in porcine expanded potential stem cells.Frontiers in cell and developmental biology · 2026Article
- Defining core signaling pathways for supporting in vitro maintenance of pig extraembryonic endoderm (XEN) cells.Reproduction (Cambridge, England) · 2025Article
- Current Status of Synthetic Mammalian Embryo Models.International journal of molecular sciences · 2024Review
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).International journal of molecular sciences · 2021Article
- Derivation of Porcine Extra-Embryonic Endoderm Cell Lines Reveals Distinct Signaling Pathway and Multipotency States.International journal of molecular sciences · 2021Article
- Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos.Stem cell reports · 2021Article
- ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.Frontiers in cell and developmental biology · 2021Article
- BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2.Cell proliferation · 2021Article
- Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone.Stem cell research & therapy · 2020Article
- Derivation of porcine extraembryonic endoderm-like cells from blastocysts.Cell proliferation · 2020Article
- Characterization of porcine extraembryonic endoderm cells.Cell proliferation · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 3 institutions in 1 country.
Funding
Abstract
objectivesTo date, many efforts have been made to establish porcine embryonic stem (pES) cells without success. Extraembryonic endoderm (XEN) cells can self-renew and differentiate into the visceral endoderm and parietal endoderm. XEN cells are derived from the primitive endoderm of the inner cell mass of blastocysts and may be an intermediate state in cell reprogramming. MATERIALS AND
methodsPorcine XEN cells (pXENCs) were generated from porcine pluripotent stem cells (pPSCs) and were characterized by RNA sequencing and immunofluorescence analyses. The developmental potential of pXENCs was investigated in chimeric mouse embryos.
resultsPorcine XEN cells derived from porcine pPSCs were successfully expanded in N2B27 medium supplemented with bFGF for least 30 passages. RNA sequencing and immunofluorescence analyses showed that pXENCs expressed the murine and canine XEN markers Gata6, Gata4, Sox17 and Pdgfra but not the pluripotent markers Oct4, Sox2 and TE marker Cdx2. Moreover, these cells contributed to the XEN when injected into four-cell stage mouse embryos. Supplementation with Chir99021 and SB431542 promoted the pluripotency of the pXENCs.
conclusionsWe successfully derived pXENCs and showed that supplementation with Chir99021 and SB431542 confer them with pluripotency. Our results provide a new resource for investigating the reprogramming mechanism of porcine-induced pluripotent stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.