ArticleOptics express2019
Coherent-hybrid STED: high contrast sub-diffraction imaging using a bi-vortex depletion beam.
Article in Optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Low kinetochore-microtubule occupancy leaves a mitotic memory by delaying checkpoint silencing.Nature communications · 2026Article
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.Nature communications · 2024Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
- Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors.Cell reports · 2021Article
- RETREG1/FAM134B mediated autophagosomal degradation of AMFR/GP78 and OPA1 -a dual organellar turnover mechanism.Autophagy · 2021Article
- Laser-Material Interactions of High-Quality Ultrashort Pulsed Vector Vortex Beams.Micromachines · 2021Article
- Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.The EMBO journal · 2020Article
- The Tubulin Code in Mitosis and Cancer.Cells · 2020Review
- Background Reduction in STED-FCS Using a Bivortex Phase Mask.ACS photonics · 2020Article
- α-Tubulin detyrosination impairs mitotic error correction by suppressing MCAK centromeric activity.The Journal of cell biology · 2020Article
- Fluorescence imaging with tailored light.Nanophotonics (Berlin, Germany) · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Stimulated emission depletion (STED) fluorescence microscopy squeezes an excited spot well below the wavelength scale using a doughnut-shaped depletion beam. To generate a doughnut, a scale-free vortex phase modulation (2D-STED) is often used because it provides maximal transverse confinement and radial-aberration immunity (RAI) to the central dip. However, RAI also means blindness to a defocus term, making the axial origin of fluorescence photons uncertain within the wavelength scale provided by the confocal detection pinhole. Here, to reduce the uncertainty, we perturb the 2D-STED phase mask so as to change the sign of the axial concavity near focus, creating a dilated dip. By providing laser depletion power, the dip can be compressed back in three dimensions to retrieve lateral resolution, now at a significantly higher contrast. We test this coherent-hybrid STED (CH-STED) mode in
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