ArticleScientific reports2019
Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- STX1B variant-specific synaptic dysfunction is associated with network hyperexcitability in human iPSC-derived neurons.EBioMedicine · 2026Article
- Baker-Gordon Syndrome-Associated Synaptotagmin-1 Mutations Reduce Synaptic Strength in Mouse Primary and Human-Induced Neuronal Culture Models.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- One-step induction of human GABAergic neurons promotes presynaptic development & synapse maturation.bioRxiv : the preprint server for biology · 2025Article
- Proteomics-based receptor-ligand matching enhances differentiation maturity of human-stem-cell-derived neurons.Stem cell reports · 2025Article
- GABAergic synaptic components are largely preserved across human and mouse neuronal models.Frontiers in cellular neuroscience · 2025Article
- Article
- Human iPSC-Derived Neurons with Reliable Synapses and Large Presynaptic Action Potentials.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- A reference human induced pluripotent stem cell line for large-scale collaborative studies.Cell stem cell · 2022Article
- Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytes.iScience · 2022Article
- The microbiota-gut-brain axis and epilepsy from a multidisciplinary perspective: Clinical evidence and technological solutions for improvement of in vitro preclinical models.Bioengineering & translational medicine · 2022Review
- Progress of Induced Pluripotent Stem Cell Technologies to Understand Genetic Epilepsy.International journal of molecular sciences · 2020Review
- Autaptic Cultures: Methods and Applications.Frontiers in synaptic neuroscience · 2020Article
- Novel Peptidomic Approach for Identification of Low and High Molecular Weight Tauopathy Peptides Following Calpain Digestion, and Primary Culture Neurotoxic Challenges.International journal of molecular sciences · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently developed technology to differentiate induced pluripotent stem cells (iPSCs) into human induced neurons (iNs) provides an exciting opportunity to study the function of human neurons. However, functional characterisations of iNs have been hampered by the reliance on mass culturing protocols which do not allow assessment of synaptic release characteristics and neuronal morphology at the individual cell level with quantitative precision. Here, we have developed for the first time a protocol to generate autaptic cultures of iPSC-derived iNs. We show that our method efficiently generates mature, autaptic iNs with robust spontaneous and action potential-driven synaptic transmission. The synaptic responses are sensitive to modulation by metabotropic receptor agonists as well as potentiation by acute phorbol ester application. Finally, we demonstrate loss of evoked and spontaneous release by Unc13A knockdown. This culture system provides a versatile platform allowing for quantitative and integrative assessment of morphophysiological and molecular parameters underlying human synaptic transmission.
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