ArticleScientific reports2019
A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Comprehensive Evaluation of Safety and Probiotic Potential of Lactiplantibacillus plantarum HY7715 Isolated from Korean kimchi.Probiotics and antimicrobial proteins · 2026Article
- Mitochondrial Protein MjEF-Tu is Secreted into Host Plants by Nematodes Eliciting Immune Signaling and Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genomic and Functional Evaluation of TwoGenes · 2024Article
- Extraintestinal pathogenicVirulence · 2022Article
- Mitochondrial fusion- and fission-related protein expression in the regulation of skeletal muscle mass.Physiological reports · 2022Article
- Roles of the Cell Surface Architecture ofFrontiers in microbiology · 2021Review
- Article
- Extracellular Vesicles Produced by Bifidobacterium longum Export Mucin-Binding Proteins.Applied and environmental microbiology · 2020Article
- Cellular maps of gastrointestinal organs: getting the most from tissue clearing.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several bacterial moonlighting proteins act as adhesion factors, which are important for bacterial colonization of the gastrointestinal (GI) tract. However, little is known about the adherence properties of moonlighting proteins in the GI tract. Here, we describe a new approach for visualizing the localization of moonlighting protein-coated fluorescent microbeads in the whole GI tract by using a tissue optical clearing method, using elongation factor Tu (EF-Tu) as an example. As a bacterial cell surface-localized protein mimic, recombinant EF-Tu from Lactobacillus reuteri was immobilized on microbeads. EF-Tu-coating promoted the interaction of the microbeads with a Caco-2 cell monolayer. Next, the microbeads were orally administered to mice. GI whole tissues were cleared in aqueous fructose solutions of increasing concentrations. At 1 h after administration, the microbeads were diffused from the stomach up to the cecum, and after 3 h, they were diffused throughout the intestinal tract. In the lower digestive tract, EF-Tu-beads were significantly more abundant than non-coated control beads, suggesting that EF-Tu plays an important role in the persistence of the microbeads in the GI tract. The new approach will help in evaluating how moonlighting proteins mediate bacterial colonization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.