Evidence map›Paper›PMID 30894430›Full record

ArticlemSphere2019

A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in

Flavia C G Reis, Beatriz S Borges, Luísa J Jozefowicz, Bianca A G Sena, Ane W A Garcia, Lia C Medeiros, Sharon T Martins, Leandro Honorato, Augusto Schrank, Marilene H Vainstein and 5 more

Open access · goldAbstract read
In one paragraph

Article in mSphere, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 108 citations in OpenAlex.

  1. Article
  2. Extracellular Vesicles fromJournal of fungi (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Extracellular vesicles ofInfection and immunity · 2026
    Article
  9. Article
  10. Article
  11. Article
  12. An anti-adhesive peptide found in extracellular vesicles fromCell surface (Amsterdam, Netherlands) · 2025
    Article
  13. Article
  14. Article
  15. Article
  16. Extracellular vesicles from DistinctACS infectious diseases · 2025
    Article
  17. Review
  18. Article
  19. Evolutionary uniqueeLife · 2025
    Article
  20. Article

11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Flavia C G ReisInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Beatriz S BorgesInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Luísa J JozefowiczInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Bianca A G SenaInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Ane W A GarciaCentro de Biotecnologia and Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Lia C MedeirosInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Sharon T MartinsInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Leandro HonoratoInstituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Augusto SchrankCentro de Biotecnologia and Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Marilene H VainsteinCentro de Biotecnologia and Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Livia KmetzschCentro de Biotecnologia and Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0003-3766-5869
Leonardo NimrichterInstituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Lysangela R AlvesInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
Charley C StaatsCentro de Biotecnologia and Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Marcio L RodriguesInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil marcio.rodrigues@fiocruz.br.ORCID 0000-0002-6081-3439
Fundação Oswaldo Cruz · BRUniversidade Federal do Rio Grande do Sul · BRUniversidade Federal do Rio de Janeiro · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of

Indexed as

Biological TransportCryptococcus gattiiCryptococcus neoformansExtracellular VesiclesFungal PolysaccharidesFungal ProteinsMicroscopy, Electron, TransmissionMycologyPolysaccharidesFungal PolysaccharidesFungal ProteinsglucuronoxylomannanPolysaccharidesCryptococcusextracellular vesiclesfungiscramblasesecretion

Identifiers

PMID30894430
PMCPMC6429041
OpenAlexW2912470717

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.