ReviewThe Journal of cell biology2019
Designing a rigorous microscopy experiment: Validating methods and avoiding bias.
Review in The Journal of cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence and practices of immunofluorescent cell image processing: a systematic review.Frontiers in cellular neuroscience · 2023Pooled it
- OpticalFlow3D - a tool for measuring amorphous motion in three-dimensional fluorescence microscopy images.Journal of cell science · 2026Article
- Persistence of mucosal CAR-T cells and inflammatory remodeling in enterocolitis associated with BCMA CAR-T cell therapy.Nature medicine · 2026Article
- Training module and assessment strategy for light microscopy skills training in a research environment.Journal of microbiology & biology education · 2026Article
- Circadian Control of Pulmonary Endothelial Signaling Occurs via the NADPH Oxidase 2-NLRP3 Pathway.Journal of biological rhythms · 2025Article
- SLC11A2 affects nutritional immunity in the gut epithelium.bioRxiv : the preprint server for biology · 2025Article
- Mitochondrial ROS trigger interorganellular signaling and prime ER processes to establish enhanced plant immunity.Science advances · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Creating and troubleshooting microscopy analysis workflows: Common challenges and common solutions.Journal of microscopy · 2024Article
- Creating and troubleshooting microscopy analysis workflows: common challenges and common solutions.ArXiv · 2024Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- Accounting for intensity variation in image analysis of large-scale multiplexed clinical trial datasets.The journal of pathology. Clinical research · 2023Article
- Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study.Communications biology · 2023Article
- Optimal sparsity allows reliable system-aware restoration of fluorescence microscopy images.Science advances · 2023Article
- Article
- A biologist's guide to planning and performing quantitative bioimaging experiments.PLoS biology · 2023Article
- Glycemic Challenge Is Associated with the Rapid Cellular Activation of the Locus Ceruleus and Nucleus of Solitary Tract: Circumscribed Spatial Analysis of Phosphorylated MAP Kinase Immunoreactivity.Journal of clinical medicine · 2023Article
- Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- PACInternational journal of molecular sciences · 2022Article
- Quality assessment in light microscopy for routine use through simple tools and robust metrics.The Journal of cell biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Images generated by a microscope are never a perfect representation of the biological specimen. Microscopes and specimen preparation methods are prone to error and can impart images with unintended attributes that might be misconstrued as belonging to the biological specimen. In addition, our brains are wired to quickly interpret what we see, and with an unconscious bias toward that which makes the most sense to us based on our current understanding. Unaddressed errors in microscopy images combined with the bias we bring to visual interpretation of images can lead to false conclusions and irreproducible imaging data. Here we review important aspects of designing a rigorous light microscopy experiment: validation of methods used to prepare samples and of imaging system performance, identification and correction of errors, and strategies for avoiding bias in the acquisition and analysis of images.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.