ArticleBMC plant biology2019
Generation of a new thermo-sensitive genic male sterile rice line by targeted mutagenesis of TMS5 gene through CRISPR/Cas9 system.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Plant Biotic Stress: Tools and Techniques for Crop Protection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Creation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing.Foods (Basel, Switzerland) · 2025Article
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Transcriptome and WGCNA reveals the potential genetic basis of photoperiod-sensitive male sterility in soybean.BMC genomics · 2025Article
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- Article
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Application of CRISPR/Cas9 system to knock out GluB gene for developing low glutelin rice mutant.Botanical studies · 2024Article
- Article
- Review
- Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement.GM crops & food · 2023Review
- CRISPR/Cas Technology Revolutionizes Crop Breeding.Plants (Basel, Switzerland) · 2023Review
- Genome engineering of disease susceptibility genes for enhancing resistance in plants.Functional & integrative genomics · 2023Review
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- Genome-Wide Identification of the Cytochrome P450 Superfamily Genes and Targeted Editing ofPlants (Basel, Switzerland) · 2023Article
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Review
- Thermo-Sensitive Genic Male Sterile Lines of Neo-Tetraploid Rice Developed through Gene Editing Technology Revealed High Levels of Hybrid Vigor.Plants (Basel, Switzerland) · 2022Article
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Authors and funding
11 authors.
Funding
Abstract
backgroundTwo-line hybrid rice with high yield potential is increasingly popular and the photo- and temperature-sensitive male sterile line is one of the basic components for two-line hybrid rice breeding. The development of male sterile lines through conventional breeding is a lengthy and laborious process, whereas developing thermo-sensitive genic male sterile (TGMS) lines for two-line hybrid breeding by editing a temperature-sensitivity gene by CRISPR/Cas9 is efficient and convenient.
resultsHere, thermo-sensitive genic male sterility (TGMS) was induced by employing the CRISPR/Cas9 gene editing technology to modify the gene TMS5. Two TGMS mutants, tms5-1 and tms5-2, both lacking any residual T-DNA, were generated in the indica rice cultivar Zhongjiazao17 (cv. YK17) background. When grown at a sub-optimal temperature (22 °C), both mutants produced viable pollen and successfully produced grain through self-fertilization, but at temperatures 24 and 26 °C, their pollen was sterile and no grain was set. F
conclusionThe YK17S generated by CRISPR/Cas9 system was proved to be a new TGMS line with superior yield potential and can be widely utilized in two-line hybrid breeding of indica rice.
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