ArticleGenes2019
H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells.
Article in Genes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Establishment and Characterization of an Immortalized Porcine Satellite Cell Line from China Junmu No.1 Pigs.Veterinary sciences · 2026Article
- Histone Modifications: Decoding the Epigenetic Basis of Economic Traits in Livestock and Poultry.Genes · 2026Review
- Biomimetic functionalized metal organic frameworks as multifunctional agents: Paving the way for cancer vaccine advances.Materials today. Bio · 2024Review
- Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes.Journal of animal science and biotechnology · 2024Article
- Article
- A PITX2-HTR1B pathway regulates the asymmetric development of female gonads in chickens.PNAS nexus · 2023Article
- LncRNAInternational journal of molecular sciences · 2023Article
- Transcriptomic Analysis Reveals mRNA and Alternative Splicing Events in Ovine Skeletal Muscle Satellite Cells during Proliferation and Differentiation.Animals : an open access journal from MDPI · 2023Article
- Long non-coding RNAEpigenetics · 2022Article
- Tcf12 is required to sustain myogenic genes synergism with MyoD by remodelling the chromatin landscape.Communications biology · 2022Article
- H3K27ac chromatin acetylation and gene expression analysis reveal sex- and situs-related differences in developing chicken gonads.Biology of sex differences · 2022Article
- Visfatin is a multifaceted molecule that exerts regulation effects on inflammation and apoptosis in RAW264.7 cells and mice immune organs.Frontiers in immunology · 2022Article
- Hypoxia in Cell Reprogramming and the Epigenetic Regulations.Frontiers in cell and developmental biology · 2021Review
- Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development.Frontiers in cell and developmental biology · 2021Article
- Comparative Analysis Among Different Species Reveals That the Androgen Receptor Regulates Chicken Follicle Selection Through Species-Specific Genes Related to Follicle Development.Frontiers in genetics · 2021Article
- Lactate Metabolism and Satellite Cell Fate.Frontiers in physiology · 2020Review
- RIP-Seq of EZH2 IdentifiesFrontiers in cell and developmental biology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine.
methodsPorcine satellite cells (PSCs) were isolated and in-vitro cultured from newborn piglets. RNA Sequencing (RNA-Seq) and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers-H3K4me3, H3K27me3, and H3K27ac-and RNA polymerase II.
resultsThe study identified 917 differentially expressed genes during cell differentiation. The landscape of epigenetic marks was displayed on a genome-wide scale, which had globally shrunken. H3K27me3 reinforcement participated in obstructing the transcription of proliferation-related genes, while its depletion was closely related to the up-regulation of myogenic genes. Furthermore, the degree of H3K27me3 modification was dramatically reduced by 50%, and 139 myogenic genes were upregulated to promote cell differentiation.
conclusionsThe depletion of H3K27me3 was shown to promote porcine satellite cell differentiation through upregulating the transcription level of myogenic genes. Our findings in this study provide new insights of the epigenomic mechanisms occurring during myogenic differentiation, and shed light on chromatin states and the dynamics underlying myogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.