ArticlePloS one2019
Isolation of B-cells using Miltenyi MACS bead isolation kits.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
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- Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells.Biomicrofluidics · 2023Article
- Selection of Cell Populations with High or Low Surface Marker Expression Using Magnetic Sorting.Cells · 2023Article
- Enrichment of Human Dermal Stem Cells from Primary Cell Cultures through the Elimination of Fibroblasts.Cells · 2023Article
- B cell receptor repertoire analysis from autopsy samples of COVID-19 patients.Frontiers in immunology · 2023Article
- Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens.STAR protocols · 2022Article
- Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting.Nature biomedical engineering · 2022Article
- Increased serum level of alpha-2 macroglobulin and its production by B-lymphocytes in chronic lymphocytic leukemia.Frontiers in immunology · 2022Article
- The red blood cell as a novel regulator of human B-cell activation.Immunology · 2021Article
- Magnetic-Activated Cell Sorting Using Coiled-Coil Peptides: An Alternative Strategy for Isolating Cells with High Efficiency and Specificity.ACS applied materials & interfaces · 2021Article
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- Crocin Promotes Apoptosis in Human EBV-Transformed B-Lymphocyte via Intrinsic Pathway.Mediterranean journal of hematology and infectious diseases · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This article describes the procedures used to isolate pure B-cell populations from whole blood using various Miltenyi magnetic-activated cell sorting (MACS) bead Isolation kits. Such populations are vital for studies investigating the functional capacity of B-cells, as the presence of other cell types may have indirect effects on B-cell function through cell-cell interactions or by secretion of several soluble molecules. B-cells can be isolated by two main approaches: 1) Negative selection-in which B-cells remain "untouched" in their native state; this is advantageous as it is likely that B-cells remain functionally unaltered by this process. 2) Positive selection-in which B-cells are labelled and actively removed from the sample. We used three Negative B-cell isolation kits as well as the Positive B-cell isolation kit from Miltenyi and compared the purity of each of the resulting B-cells fractions. Contamination of isolated B-cell fractions with platelets was the conclusive finding for all of the isolation techniques tested. These results illustrate the inefficiency of current available MACS B-cell isolation kits to produce pure B-cell populations, from which concrete findings can be made. As such we suggest cell sorting as the preferred method for isolating pure B-cells to be used for downstream functional assays.
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