Evidence map›Paper›PMID 30892819›Full record

ArticleJournal of diabetes investigation2019

Conditioned media from dental pulp stem cells improved diabetic polyneuropathy through anti-inflammatory, neuroprotective and angiogenic actions: Cell-free regenerative medicine for diabetic polyneuropathy.

Eriko Makino, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Saki Kanada, Masaki Hata, Tomokazu Saiki, Kazunori Sango, Hideki Kamiya, Jiro Nakamura and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes investigation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 43 citations in OpenAlex.

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  19. Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Eriko MakinoDepartment of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Nobuhisa NakamuraDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Megumi MiyabeDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Mizuho ItoDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Saki KanadaDepartment of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Masaki HataDepartment of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Tomokazu SaikiDepartment of Pharmacy, Dental Hospital, Aichi Gakuin University, Nagoya, Japan.
Kazunori SangoLaboratory of Peripheral Nerve Pathophysiology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Hideki KamiyaDivision of Diabetes, Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.
Jiro NakamuraDivision of Diabetes, Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.
Ken MiyazawaDepartment of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Shigemi GotoDepartment of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Tatsuaki MatsubaraDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Keiko NaruseDepartment of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.ORCID https://orcid.org/0000-0002-0062-0694
Aichi Gakuin University · JPAichi Medical University · JPTokyo Metropolitan Institute of Medical Science · JP

Funding

Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT). 21592506
6 · The paper itself

Abstract

AIMS/

introductionDental pulp stem cells (DPSCs) can be easily obtained from teeth for general orthodontic reasons. We have previously reported the therapeutic effects of DPSC transplantation for diabetic polyneuropathy. As abundant secretomes from DPSCs are considered to play a central role in the improvement of diabetic polyneuropathy, we investigated whether direct injection of DPSC-conditioned media (DPSC-CM) into hindlimb skeletal muscles ameliorates diabetic polyneuropathy in diabetic rats. MATERIALS AND

methodsDPSCs were isolated from the dental pulp of Sprague-Dawley rats. Eight weeks after the induction of diabetes, DPSC-CM was injected into the unilateral hindlimb skeletal muscles in both normal and diabetic rats. The effects of DPSC-CM on diabetic polyneuropathy were assessed 4 weeks after DPSC-CM injection. To confirm the angiogenic effect of DPSC-CM, the effect of DPSC-CM on cultured human umbilical vascular endothelial cell proliferation was investigated.

resultsThe administration of DPSC-CM into the hindlimb skeletal muscles significantly ameliorated sciatic motor/sensory nerve conduction velocity, sciatic nerve blood flow and intraepidermal nerve fiber density in the footpads of diabetic rats. We also showed that DPSC-CM injection significantly increased the capillary density of the skeletal muscles, and suppressed pro-inflammatory reactions in the sciatic nerves of diabetic rats. Furthermore, an in vitro study showed that DPSC-CM significantly increased the proliferation of umbilical vascular endothelial cells.

conclusionsWe showed that DPSC-CM injection into hindlimb skeletal muscles has a therapeutic effect on diabetic polyneuropathy through neuroprotective, angiogenic and anti-inflammatory actions. DPSC-CM could be a novel cell-free regenerative medicine treatment for diabetic polyneuropathy.

Indexed as

Regenerative MedicineAngiogenesis Inducing AgentsAnimalsAnti-Inflammatory AgentsCell ProliferationCulture Media, ConditionedDental PulpDiabetes Mellitus, ExperimentalDiabetic NeuropathiesHumansHuman Umbilical Vein Endothelial CellsMaleMuscle, SkeletalNeural ConductionNeuroprotective AgentsRatsAngiogenesis Inducing AgentsAnti-Inflammatory AgentsCulture Media, ConditionedNeuroprotective AgentsDental pulp stem cellDiabetic neuropathyRegenerative medicine

Identifiers

PMID30892819
PMCPMC6717901
OpenAlexW2924591369

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.