ArticleAngewandte Chemie (International ed. in English)2019
Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.
Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish.The EMBO journal · 2024Article
- Oxidative Stress, Transfer RNA Metabolism, and Protein Synthesis.Antioxidants & redox signaling · 2024Review
- Advances in methods for tRNA sequencing and quantification.Trends in genetics : TIG · 2024Review
- Non-covalent dyes in microscale thermophoresis for studying RNA ligand interactions and modifications.Chemical science · 2023Article
- RNA marker modifications reveal the necessity for rigorous preparation protocols to avoid artifacts in epitranscriptomic analysis.Nucleic acids research · 2022Article
- Targeted genome editing of plants and plant cells for biomanufacturing.Transgenic research · 2021Review
- High-resolution quantitative profiling of tRNA abundance and modification status in eukaryotes by mim-tRNAseq.Molecular cell · 2021Article
- Numerical Analysis of Thermophoresis of a Charged Spheroidal Colloid in Aqueous Media.Micromachines · 2021Article
- Article
- Combining tRNA sequencing methods to characterize plant tRNA expression and post-transcriptional modification.RNA biology · 2021Article
- TheRNA (New York, N.Y.) · 2020Article
- Article
- Misactivation of multiple starvation responses in yeast by loss of tRNA modifications.Nucleic acids research · 2020Article
- Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.Angewandte Chemie (International ed. in English) · 2019Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
Abstract
Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.