ArticleACS applied materials & interfaces2019
Bio-Inspired NanoVilli Chips for Enhanced Capture of Tumor-Derived Extracellular Vesicles: Toward Non-Invasive Detection of Gene Alterations in Non-Small Cell Lung Cancer.
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Article in ACS applied materials & interfaces, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Bioorthogonal Click Chemistry-Enabled Enrichment of Extracellular Vesicles for Integrated Molecular and Functional Liquid Biopsy§.Accounts of chemical research · 2026Article
- Tumor Extracellular Vesicle Digital Scoring Assays for Cancer Detection and Monitoring.Precision chemistry · 2025Review
- Bridging Discovery and Treatment: Cancer Biomarker.Cancers · 2025Review
- Noninvasive Assessment of Protease Activity in Osteosarcoma via Click Chemistry-Mediated Enrichment of Extracellular Vesicles.Advanced functional materials · 2025Article
- Efficient methods of isolation and purification of extracellular vesicles.Nano convergence · 2025Review
- Noninvasive Assessment of β-Secretase Activity Through Click Chemistry-Mediated Enrichment of Neuronal Extracellular Vesicles to Detect Alzheimer's Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Tumor-derived systems as novel biomedical tools-turning the enemy into an ally.Biomaterials research · 2023Review
- Assessing Breast Cancer Molecular Subtypes Using Extracellular Vesicles' mRNA.Analytical chemistry · 2023Article
- Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis.Pharmaceutics · 2023Review
- Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2023Review
- Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications.Biosensors · 2022Review
- Nano-omics: nanotechnology-based multidimensional harvesting of the blood-circulating cancerome.Nature reviews. Clinical oncology · 2022Review
- Microfluidics for detection of exosomes and microRNAs in cancer: State of the art.Molecular therapy. Nucleic acids · 2022Review
- Coupling Lipid Labeling and Click Chemistry Enables Isolation of Extracellular Vesicles for Noninvasive Detection of Oncogenic Gene Alterations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Small extracellular vesicles in cancer.Bioactive materials · 2021Review
- Advanced Nanotechnologies for Extracellular Vesicle-Based Liquid Biopsy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Advances in microfluidic extracellular vesicle analysis for cancer diagnostics.Lab on a chip · 2021Review
- Current Search through Liquid Biopsy of Effective Biomarkers for Early Cancer Diagnosis into the Rich Cargoes of Extracellular Vesicles.International journal of molecular sciences · 2021Review
- Epidermal Growth Factor Receptor Mutations Carried in Extracellular Vesicle-Derived Cargo Mirror Disease Status in Metastatic Non-small Cell Lung Cancer.Frontiers in cell and developmental biology · 2021Article
- Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes.International journal of nanomedicine · 2021Review
Corrections and comments
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Authors and funding
34 authors at 8 institutions in 3 countries.
Jiantong DongBeijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , P. R. China.ORCID http://orcid.org/0000-0001-7063-711X
Ryan Y ZhangORCID http://orcid.org/0000-0002-3122-4053
Na SunKey Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics , Chinese Academy of Sciences , Suzhou 215123 , P. R. China.ORCID http://orcid.org/0000-0002-3282-4405
Matthew SmalleyORCID http://orcid.org/0000-0002-0509-6502
Shih-Jie ChouInstitute of Pharmacology , National Yang-Ming University , Taipei 112 , Taiwan.ORCID http://orcid.org/0000-0003-2610-8178
Yu Jen JanSamuel Oschin Comprehensive Cancer Institute , Cedars-Sinai Medical Center , Los Angeles , California 90048 , United States.ORCID http://orcid.org/0000-0002-2962-2933
Dongping QiORCID http://orcid.org/0000-0003-1624-8142
Xinghong TangORCID http://orcid.org/0000-0002-2342-5058
Patrick TsengORCID http://orcid.org/0000-0002-6536-5647
Rueihung KaoORCID http://orcid.org/0000-0001-8541-2693
Meng MengState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research , Nankai University , Haihe Education Park, 38 Tongyan Road , Tianjin 300353 , P. R. China.ORCID http://orcid.org/0000-0002-9715-6292
Xirun ZhengDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0003-3342-3256
Ying LiuDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0002-0734-8873
Tatyana VagnerDivision of Cancer Biology and Therapeutics, Departments of Surgery, Biomedical Sciences and Pathology and Laboratory Medicine , Samuel Oschin Comprehensive Cancer Institute , Los Angeles , California 90048 , United States.ORCID http://orcid.org/0000-0003-0258-8250
Xiaoshu ChaiDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0002-6459-6558
Dongjing ZhouDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0003-1172-8681
Mengyuan LiBeijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , P. R. China.ORCID http://orcid.org/0000-0002-4664-1845
Shih-Hwa ChiouInstitute of Pharmacology , National Yang-Ming University , Taipei 112 , Taiwan.ORCID http://orcid.org/0000-0002-8651-5755
Guangjuan ZhengDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0001-5570-2470
Dolores Di VizioDivision of Cancer Biology and Therapeutics, Departments of Surgery, Biomedical Sciences and Pathology and Laboratory Medicine , Samuel Oschin Comprehensive Cancer Institute , Los Angeles , California 90048 , United States.ORCID http://orcid.org/0000-0002-9787-6556
Vatche G AgopianORCID http://orcid.org/0000-0003-0130-9838
Edwin PosadasSamuel Oschin Comprehensive Cancer Institute , Cedars-Sinai Medical Center , Los Angeles , California 90048 , United States.ORCID http://orcid.org/0000-0001-8649-1346
Steven J JonasORCID http://orcid.org/0000-0002-8111-0249
Shin-Pon JuDepartment of Mechanical and Electro-Mechanical Engineering , National Sun Yat-Sen University , Kaohsiung 80424 , Taiwan.ORCID http://orcid.org/0000-0002-9454-8899
Paul S WeissORCID http://orcid.org/0000-0001-5527-6248
Meiping ZhaoBeijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , P. R. China.ORCID http://orcid.org/0000-0002-8696-8738
Hsian-Rong TsengORCID http://orcid.org/0000-0003-0942-5905
Yazhen ZhuDepartment of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences , Guangzhou 510120 , P. R. China.ORCID http://orcid.org/0000-0002-2130-8085
California NanoSystems Institute · USGuangzhou University of Chinese Medicine · CNCedars-Sinai Medical Center · USPeking University · CNSamueli Institute · USNational Sun Yat-sen University · TWNational Yang Ming Chiao Tung University · TWUniversity of California, Los Angeles · US
Funding
The Effect of Nanoparticle Encapsulated Resveratrol on Treatment of PeriodontitisKL2TR001882 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Elizabeta Nemeth · 2016 to 2026
$13.1MHCC EV Digital Scoring Assay for assessing treatment response in HCC patientsR01CA253651 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TSENG, HSIAN-RONG, ZHU, YAZHEN · 2020 to 2024
$3.3MA blood test to identify prostate cancer patients at risk for visceral metastasisR01CA218356 · NCI · CEDARS-SINAI MEDICAL CENTER · PI POSADAS, EDWIN MELENCIO, TSENG, HSIAN-RONG · 2017 to 2021
$3.0MIntegrated analysis of HCC CTCs for Liver Transplant Candidate SelectionR01CA246304 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2024
$2.8MThermoresponsive NanoVelcro CTC Purification System for Prostate Cancer ProfilingU01CA198900 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI POSADAS, EDWIN MELENCIO, TSENG, HSIAN-RONG · 2015 to 2019
$2.4MMolecular and Functional Analysis of Single Circulating Melanoma CellsR33CA174562 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TSENG, HSIAN-RONG · 2013 to 2015
$987kClick Chemistry-Mediated Microfluidic Sorting for HCC CTCsR21CA235340 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2019 to 2021
$543kAnalysis of HCC CTC Phenotype for Liver Transplant Candidate SelectionR21CA216807 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE · 2018 to 2019
$368kNCATS NIH HHS KL2 TR001882NCI NIH HHS R01 CA218356NCI NIH HHS R01 CA246304NCI NIH HHS R01 CA253651NCI NIH HHS R21 CA216807NCI NIH HHS R21 CA235340NCI NIH HHS R33 CA174562NCI NIH HHS U01 CA198900
Abstract
Tumor-derived extracellular vesicles (EVs) present in bodily fluids are emerging liquid biopsy markers for non-invasive cancer diagnosis and treatment monitoring. Because the majority of EVs in circulation are not of tumor origin, it is critical to develop new platforms capable of enriching tumor-derived EVs from the blood. Herein, we introduce a biostructure-inspired NanoVilli Chip, capable of highly efficient and reproducible immunoaffinity capture of tumor-derived EVs from blood plasma samples. Anti-EpCAM-grafted silicon nanowire arrays were engineered to mimic the distinctive structures of intestinal microvilli, dramatically increasing surface area and enhancing tumor-derived EV capture. RNA in the captured EVs can be recovered for downstream molecular analyses by reverse transcription Droplet Digital PCR. We demonstrate that this assay can be applied to monitor the dynamic changes of ROS1 rearrangements and epidermal growth factor receptor T790M mutations that predict treatment responses and disease progression in non-small cell lung cancer patients.
Indexed as
AdultAgedAntibodies, ImmobilizedBiomarkers, TumorCarcinoma, Non-Small-Cell LungEpithelial Cell Adhesion MoleculeErbB ReceptorsExtracellular VesiclesFemaleGene RearrangementHumansLung NeoplasmsMaleMiddle AgedNanowiresPolymerase Chain ReactionAntibodies, ImmobilizedBiomarkers, TumorEpithelial Cell Adhesion MoleculeErbB ReceptorsProtein-Tyrosine KinasesProto-Oncogene ProteinsRNA, MessengerROS1 protein, humanSiliconEGFR T790M mutationextracellular vesiclesmicrofluidicsnanosubstratesnon-small cell lung cancerROS1 rearrangements
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