Evidence map›Paper›PMID 30890899›Full record

ArticleClinical proteomics2019

Rapid detection of colistin resistance protein MCR-1 by LC-MS/MS.

Honghui Wang, Yong Chen, Jeffrey R Strich, Steven K Drake, Jung-Ho Youn, Avi Z Rosenberg, Marjan Gucek, Patrick T McGann, Anthony F Suffredini, John P Dekker

Abstract read
In one paragraph

Article in Clinical proteomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Proteomic assay for rapid characterisation of Staphylococcus aureus antimicrobial resistance mechanisms directly from blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Early and specific targeted mass spectrometry-based identification of bacteria in endotracheal aspirates of patients suspected with ventilator-associated pneumonia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021
    Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Honghui Wang1Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD USA.
Yong Chen2Proteomics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD USA.
Jeffrey R Strich1Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD USA.
Steven K Drake1Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD USA.
Jung-Ho Youn3Department of Laboratory Medicine, Clinical Center, Microbiology Service, National Institutes of Health, 10 Center Drive, Bethesda, MD USA.
Avi Z Rosenberg4Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD USA.
Marjan Gucek2Proteomics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD USA.
Patrick T McGann6Walter Reed Army Institute of Research, Silver Spring, MD USA.
Anthony F Suffredini1Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD USA.
John P Dekker3Department of Laboratory Medicine, Clinical Center, Microbiology Service, National Institutes of Health, 10 Center Drive, Bethesda, MD USA.

Funding

Proteomics CoreZICHL005903 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI GUCEK, MARJAN · 2009 to 2022
$23.3M
Novel Applications of Mass Spectrometry and Proteomics to Infectious Disease DiagnosticsZIAAI001252 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DEKKER, JOHN · 2019 to 2020
$742k
Diagnostic and Prognostic Biomarkers in PneumoniaZIACL008063 · CLC · CLINICAL CENTER · PI SUFFREDINI, ANTHONY F. · 2009 to 2025
$0k
6 · The paper itself

Abstract

backgroundColistin (polymyxin E) and polymixin B are important bactericidal antibiotics used in the treatment of serious infections caused by multi-drug resistant Gram-negative organisms. Transferrable plasmid-mediated colistin resistance, conferred by the product of the

methodsMCR-1 tryptic peptides that were efficiently ionized and readily detectable were characterized in a set of

resultsAll of the

conclusionsThis work demonstrates the feasibility of rapid detection of the MCR-1 protein in bacterial isolates by LC-MS/MS.

Identifiers

PMID30890899
PMCPMC6390366

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.